Abstract：
Cellular technologies are one of the most promising areas of biomedicine, which is based on the isolation of cells of various types, followed by their cultivation and use, or the use of their metabolic products, for medical purposes. Today, a significant part of biomedical research is carried out in vitro. On the other hand, organotypic culture can be used as a powerful model system and can complement cell culture and in vivo studies in different biomedical applications. Uterine leiomyoma (UL) is a very common benign tumor and often leads to many reproductive complications. Herein we describe a fast and reliable method of isolation and UL primary cells culturing along with the development of a UL organotypic model. We propose the usage of UL primary cells in experimental work at a first passage to prevent loss of driver mutations in MED12 and HMGA2 genes. New optimized conditions for the growth and maintenance of 2D and 3D models of uterine leiomyoma in vitro are suggested.
摘要：
细胞技术是生物医学最有前途的领域之一，这是基于各种类型细胞的分离，其次是他们的种植和使用，或者使用它们的代谢产物，用于医疗目的。今天,生物医学研究的重要部分是在体外进行的。另一方面,器官型培养可以用作一个强大的模型系统，可以补充不同生物医学应用中的细胞培养和体内研究。子宫平滑肌瘤（UL）是一种非常常见的良性肿瘤，通常会导致许多生殖并发症。在本文中，我们描述了一种快速可靠的分离和UL原代细胞培养方法，以及UL器官型模型的开发。我们建议在第一次传代的实验工作中使用UL原代细胞来防止MED12和HMGA2基因中驱动突变的丢失。提出了新的优化条件，用于体外生长和维持子宫平滑肌瘤的2D和3D模型。
