Abstract：
OBJECTIVE: We aimed to investigate RF-EMR-induced cell malignant transformation.
METHODS: We divided Balb/c-3T3 cells into sham and expo groups. The expo groups were exposed to a 1800 MHz RF continuous wave for 40 and 60 days, for 4 h per day. The sham group was sham-exposed. Cells were harvested for a cell transformation assay, transplantation in severe combined immune deficient (SCID) mice, soft agar clone formation detection, and a transwell assay. The mRNA microarray assay was used to declare key genes and pathways.
RESULTS: The exposed Balb/c-3T3 cells showed a strong increase in cell proliferation and migration. Malignant transformation was observed in expo Balb/c-3T3 cells exposed for 40 days and 60 days, which was symbolized with visible foci and clone formation. Expo Balb/c-3T3 cells that were exposed for 40 days and 60 days produced visible tumors in the SCID mice. Lipid metabolism was the key biological process and pathway involved. The mevalonate (MVA) pathway was the key metabolic pathway. The interacted miRNAs could be further research targets to examine the molecular mechanism of the carcinogenic effects of long-term exposure.
CONCLUSIONS: Exposure for 40 and 60 days to 1800 MHz RF-EMR induced malignant transformation in Balb/c-3T3 cells at the SAR of 8.0 W/kg. We declared that lipid metabolism was the pivotal biological process and pathway. The MVA pathway was the key metabolic pathway.
METHODS: We divided Balb/c-3T3 cells into sham and expo groups. The expo groups were exposed to a 1800 MHz RF continuous wave for 40 and 60 days, for 4 h per day. The sham group was sham-exposed. Cells were harvested for a cell transformation assay, transplantation in severe combined immune deficient (SCID) mice, soft agar clone formation detection, and a transwell assay. The mRNA microarray assay was used to declare key genes and pathways.
RESULTS: The exposed Balb/c-3T3 cells showed a strong increase in cell proliferation and migration. Malignant transformation was observed in expo Balb/c-3T3 cells exposed for 40 days and 60 days, which was symbolized with visible foci and clone formation. Expo Balb/c-3T3 cells that were exposed for 40 days and 60 days produced visible tumors in the SCID mice. Lipid metabolism was the key biological process and pathway involved. The mevalonate (MVA) pathway was the key metabolic pathway. The interacted miRNAs could be further research targets to examine the molecular mechanism of the carcinogenic effects of long-term exposure.
CONCLUSIONS: Exposure for 40 and 60 days to 1800 MHz RF-EMR induced malignant transformation in Balb/c-3T3 cells at the SAR of 8.0 W/kg. We declared that lipid metabolism was the pivotal biological process and pathway. The MVA pathway was the key metabolic pathway.
摘要：
目的：我们旨在研究RF-EMR诱导的细胞恶性转化。
方法：我们将Balb/c-3T3细胞分为假手术组和实验组。博览会组暴露于1800MHz的射频连续波40天和60天，每天4小时。假手术组进行假暴露。收获细胞用于细胞转化测定,严重联合免疫缺陷（SCID）小鼠的移植，软琼脂克隆形成检测，和transwell分析。mRNA微阵列测定用于声明关键基因和途径。
结果：暴露的Balb/c-3T3细胞显示出细胞增殖和迁移的强烈增加。在暴露40天和60天的expoBalb/c-3T3细胞中观察到恶性转化，以可见的病灶和克隆形成为象征。暴露40天和60天的ExpoBalb/c-3T3细胞在SCID小鼠中产生可见的肿瘤。脂质代谢是涉及的关键生物学过程和途径。甲羟戊酸（MVA）途径是关键的代谢途径。相互作用的miRNA可以作为进一步研究的目标，以检查长期暴露的致癌作用的分子机制。
结论：暴露于1800MHzRF-EMR40和60天，在SAR为8.0W/kg的Balb/c-3T3细胞中诱导恶性转化。我们宣称脂质代谢是关键的生物学过程和途径。MVA途径是关键的代谢途径。
方法：我们将Balb/c-3T3细胞分为假手术组和实验组。博览会组暴露于1800MHz的射频连续波40天和60天，每天4小时。假手术组进行假暴露。收获细胞用于细胞转化测定,严重联合免疫缺陷（SCID）小鼠的移植，软琼脂克隆形成检测，和transwell分析。mRNA微阵列测定用于声明关键基因和途径。
结果：暴露的Balb/c-3T3细胞显示出细胞增殖和迁移的强烈增加。在暴露40天和60天的expoBalb/c-3T3细胞中观察到恶性转化，以可见的病灶和克隆形成为象征。暴露40天和60天的ExpoBalb/c-3T3细胞在SCID小鼠中产生可见的肿瘤。脂质代谢是涉及的关键生物学过程和途径。甲羟戊酸（MVA）途径是关键的代谢途径。相互作用的miRNA可以作为进一步研究的目标，以检查长期暴露的致癌作用的分子机制。
结论：暴露于1800MHzRF-EMR40和60天，在SAR为8.0W/kg的Balb/c-3T3细胞中诱导恶性转化。我们宣称脂质代谢是关键的生物学过程和途径。MVA途径是关键的代谢途径。
