Abstract:
Rapid and accurate food pathogen detection is an essential step to preventing foodborne illnesses. Before detection, removal of bacteria from the food matrix and concentration to detectable levels are often essential steps. Although many reviews discuss rapid concentration methods for foodborne pathogens, the use of glycan-coated magnetic nanoparticles (MNPs) is often omitted. This review seeks to analyze the potential of this technique as a rapid and cost-effective solution for concentration of bacteria directly from foods. The primary focus is the mechanism of glycan-coated MNP binding, as well as its current applications in concentration of foodborne pathogens. First, a background on the synthesis, properties, and applications of MNPs is provided. Second, synthesis of glycan-coated particles and their theorized mechanism for bacterial adhesion is described. Existing research into extraction of bacteria directly from food matrices is also analyzed. Finally, glycan-coated MNPs are compared to the magnetic separation technique of immunomagnetic separation (IMS) in terms of cost, time, and other factors. At its current state, glycan-coated MNPs require more research to fully identify the mechanism, potential for optimization, and extraction capabilities directly in food matrices. However, current research indicates glycan-coated MNPs are an incredibly cost-effective method for rapid food pathogen extraction and concentration.
摘要:
快速准确的食品病原体检测是预防食源性疾病的重要步骤。在检测之前,从食物基质中去除细菌并将其浓缩至可检测的水平通常是必不可少的步骤。尽管许多评论讨论了食源性病原体的快速浓缩方法,通常省略聚糖包被的磁性纳米颗粒(MNPs)的使用。这篇综述旨在分析该技术作为一种快速且具有成本效益的解决方案的潜力,用于直接从食物中浓缩细菌。主要的焦点是聚糖包被的MNP结合的机制,以及其目前在食源性病原体浓缩中的应用。首先,合成的背景,属性,提供了MNPs的应用。第二,描述了聚糖涂层颗粒的合成及其细菌粘附的理论机理。还分析了直接从食品基质中提取细菌的现有研究。最后,在成本方面,将聚糖包被的MNPs与免疫磁性分离(IMS)的磁性分离技术进行了比较,时间,和其他因素。在目前的状态下,聚糖包被的MNPs需要更多的研究来充分识别机制,优化的潜力,和直接在食物基质中的提取能力。然而,目前的研究表明,聚糖包被的MNPs是一种非常具有成本效益的快速食品病原体提取和浓缩方法。