Abstract：
Fatty acid-binding proteins (FABPs) are lipid chaperones that mediate the intracellular dynamics of the hydrophobic molecules that they physically bind to. FABPs are implicated in sleep and psychiatric disorders, as well as in various cellular processes, such as cell proliferation and survival. FABP is well conserved in insects, and Drosophila has one FABP ortholog, dFabp, in its genome. Although dFabp appears to be evolutionarily conserved in some brain functions, little is known about its development and physiological function. In the present study, we investigated the function of dFabp in Drosophila development and behavior. Knockdown or overexpression of dFabp in the developing brain, wing, and eye resulted in developmental defects, such as decreased survival, altered cell proliferation, and increased apoptosis. Glia-specific knockdown of dFabp affected neuronal development, and neuronal regulation of dFabp affected glial cell proliferation. Moreover, the behavioral phenotypes (circadian rhythm and locomotor activity) of flies with regulated dFabp expression in glia and flies with regulated dFabp expression in neurons were very similar. Collectively, our results suggest that dFabp is involved in the development of various tissues and brain functions to control behavior and is a mediator of neuron-glia interactions in the Drosophila nervous system.
摘要：
脂肪酸结合蛋白(FABP)是介导与其物理结合的疏水分子的细胞内动力学的脂质伴侣。FABP与睡眠和精神疾病有关，以及在各种细胞过程中，如细胞增殖和存活。FABP在昆虫体内保存良好,果蝇有一个FABP直系同源,dFabp,在它的基因组中。尽管dFabp在某些大脑功能上似乎在进化上是保守的，对其发育和生理功能知之甚少。在本研究中,我们研究了dFabp在果蝇发育和行为中的作用。dFabp在发育中的大脑中敲除或过表达，机翼,眼睛导致了发育缺陷，比如存活率下降，改变细胞增殖，和增加细胞凋亡。dFabp的胶质细胞特异性敲除影响神经元发育，dFabp的神经元调节影响神经胶质细胞增殖。此外，神经胶质中dFabp表达受调节的果蝇和神经元中dFabp表达受调节的果蝇的行为表型（昼夜节律和运动活动）非常相似。总的来说，我们的结果表明dFabp参与各种组织的发育和大脑功能以控制行为，并且是果蝇神经系统中神经元-神经胶质相互作用的介质。
