Abstract：
Turbo cornutus, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from T. cornutus viscera (TVE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and zebrafish embryos in vivo. TVE reduced the production of LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) without any toxic effects. TVE also decreased the protein expression of LPS-induced inducible NO synthase and cyclooxygenase-2 and suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, and IL-1β. Furthermore, mechanistic studies indicated that TVE suppressed c-Jun N-terminal kinase phosphorylation and nuclear factor-kB activation. In zebrafish embryos, TVE did not show developmental toxicity based on the survival rate and cell death findings. In LPS-stimulated zebrafish embryos, TVE suppressed NO production and cell death. In conclusion, the result from this preliminary study showed TVE has a potential anti-inflammatory property that can be exploited as a functional food ingredient.
摘要：
涡轮cornutus,有角的头巾海蜗牛，沿着潮间带和玄武岩海岸线发现，是济州岛的重要渔业资源。在这项研究中,我们对从T.cornutus内脏（TVE）获得的70％乙醇提取物对脂多糖（LPS）刺激的体外RAW264.7细胞和体内斑马鱼胚胎的抗炎作用进行了初步研究。TVE减少了LPS刺激的一氧化氮（NO）和前列腺素E2（PGE2）的产生，而没有任何毒性作用。TVE还降低了LPS诱导的诱导型NO合酶和环氧合酶-2的蛋白表达，并抑制了促炎细胞因子的产生，包括肿瘤坏死因子-α,白细胞介素（IL）-6和IL-1β。此外，机制研究表明，TVE抑制c-Jun氨基末端激酶磷酸化和核因子kB激活。在斑马鱼胚胎中,根据存活率和细胞死亡发现，TVE未显示发育毒性。在LPS刺激的斑马鱼胚胎中，TVE抑制NO产生和细胞死亡。总之,这项初步研究的结果表明，TVE具有潜在的抗炎特性，可以用作功能性食品成分。
