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Abstract：
BACKGROUND: The differences in protein expression of calcium sensitive receptor (CaSR) and claudin-14 in a kidney stone model established by nanobacteria (NB) and ethylene glycol (EG) were compared.
METHODS: Ninety Wistar male rats were randomly divided into the NB group, the EG group, and the blank control group (NC group), with 30 rats in each group. Three rats of each group were sacrificed every week after injection. Histopathology was used to evaluate the stone formation of each group. The expression of CaSR and claudin-14 protein was detected by immunohistochemistry every week.
RESULTS: There was formation of bright crystals in the kidneys of the EG group and the NB group, but not the NC group. At the 3rd week, the expression of CaSR and claudin-14 in the kidney tissue of the EG group began to increase while that in the NB group increased at the 4th week. The expression of CaSR and claudin-14 protein in the EG group was stronger than that in the NB group. Meanwhile, CaSR was expressed in the NC group but did not change significantly. Claudin-14 was not expressed in the NC group.
CONCLUSIONS: Our results indicate that the traditional EG kidney stone modeling method is more rapid than the NB kidney stone modeling method, with a high stone formation rate, and the CaSR and claudin-14 protein expression levels are higher. Meanwhile, the NB used to establish the kidney stone model was isolated from patients with kidney stones, which may imitate the process of natural formation of kidney stones of patients. Therefore, the results of our research are more conducive to related research on the etiology of stones.
METHODS: Ninety Wistar male rats were randomly divided into the NB group, the EG group, and the blank control group (NC group), with 30 rats in each group. Three rats of each group were sacrificed every week after injection. Histopathology was used to evaluate the stone formation of each group. The expression of CaSR and claudin-14 protein was detected by immunohistochemistry every week.
RESULTS: There was formation of bright crystals in the kidneys of the EG group and the NB group, but not the NC group. At the 3rd week, the expression of CaSR and claudin-14 in the kidney tissue of the EG group began to increase while that in the NB group increased at the 4th week. The expression of CaSR and claudin-14 protein in the EG group was stronger than that in the NB group. Meanwhile, CaSR was expressed in the NC group but did not change significantly. Claudin-14 was not expressed in the NC group.
CONCLUSIONS: Our results indicate that the traditional EG kidney stone modeling method is more rapid than the NB kidney stone modeling method, with a high stone formation rate, and the CaSR and claudin-14 protein expression levels are higher. Meanwhile, the NB used to establish the kidney stone model was isolated from patients with kidney stones, which may imitate the process of natural formation of kidney stones of patients. Therefore, the results of our research are more conducive to related research on the etiology of stones.
摘要：
背景：比较了纳米细菌（NB）和乙二醇（EG）建立的肾结石模型中钙敏感受体（CaSR）和claudin-14蛋白表达的差异。
方法：90只雄性Wistar大鼠随机分为NB组，EG组,空白对照组（NC组），每组30只大鼠。每组3只大鼠注射后每周处死。采用组织病理学评价各组结石形成。每周用免疫组化法检测CaSR和claudin-14蛋白的表达。
结果：在EG组和NB组的肾脏中形成了明亮的晶体，但不是NC组。在第三周，EG组肾组织中CaSR和claudin-14的表达在第4周开始增加，而NB组肾组织中CaSR和claudin-14的表达在第4周开始增加。EG组CaSR和claudin-14卵白的表达强于NB组。同时,CaSR在NC组中有表达，但没有明显变化。Claudin-14在NC组中不表达。
结论：我们的结果表明，传统的EG肾结石建模方法比NB肾结石建模方法更快速，结石形成率高，CaSR和claudin-14蛋白表达水平较高。同时,用于建立肾结石模型的NB分离自肾结石患者，可以模仿患者自然形成肾结石的过程。因此,我们的研究结果更有利于结石病因的相关研究。
方法：90只雄性Wistar大鼠随机分为NB组，EG组,空白对照组（NC组），每组30只大鼠。每组3只大鼠注射后每周处死。采用组织病理学评价各组结石形成。每周用免疫组化法检测CaSR和claudin-14蛋白的表达。
结果：在EG组和NB组的肾脏中形成了明亮的晶体，但不是NC组。在第三周，EG组肾组织中CaSR和claudin-14的表达在第4周开始增加，而NB组肾组织中CaSR和claudin-14的表达在第4周开始增加。EG组CaSR和claudin-14卵白的表达强于NB组。同时,CaSR在NC组中有表达，但没有明显变化。Claudin-14在NC组中不表达。
结论：我们的结果表明，传统的EG肾结石建模方法比NB肾结石建模方法更快速，结石形成率高，CaSR和claudin-14蛋白表达水平较高。同时,用于建立肾结石模型的NB分离自肾结石患者，可以模仿患者自然形成肾结石的过程。因此,我们的研究结果更有利于结石病因的相关研究。
