The aim of this study was to assess the contribution of REEP6 to the thermogenic activation of adipocytes and characterize the metabolic consequences of REEP6 deficiency in vivo.
Expression levels of Reep6 in adipose tissue were examined by using public transcriptomic data and validated by Western blot and qPCR analyses. Adipocyte-specific regulatory roles of REEP6 were investigated in vitro in C3H10T1/2 adipocytes and in primary adipocytes obtained from REEP6 KO mice. Effects of in vivo REEP6 deficiency on energy expenditure were measured by indirect calorimetry. Mitochondrial content in adipose tissue was accessed by immunoblot, mitochondrial DNA analysis, and confocal and electron microscopy. Effects of REEP6 KO on obesity-induced metabolic dysfunction were tested in a high-fat diet-induced obesity mouse model by glucose tolerance test, Western blot, and histological analyses.
REEP6 expression is highly enriched in murine adipocytes and is sharply upregulated upon adipocyte differentiation and by cold exposure. Inactivation of REEP6 in mice increased adiposity, and reduced energy expenditure and cold tolerance. REEP6 KO severely reduced protein kinase A-mediated signaling in BAT and greatly reduced mitochondrial mass. The effect of REEP6 inactivation on diminished β-adrenergic signaling was reproduced in cultured adipocytes, indicating that this effect is cell-autonomous. REEP6 KO also suppressed expression of adenylate cyclase 3 (Adcy3) in brown adipose tissue and knockdown of REEP6 in adipocytes reduced targeting of ADCY3 to the plasma membrane. Lastly, REEP6 KO exacerbated high-fat diet-induced insulin resistance and inflammation in adipose tissue.
This study indicates that REEP6 plays an important role in β-adrenergic signal transduction in adipocytes involving the expression and trafficking of Adcy3. Genetic inactivation of REEP6 reduces energy expenditure, increases adiposity, and the susceptibility to obesity-related metabolic dysfunction.
本研究的目的是评估REEP6对脂肪细胞产热活化的贡献,并表征REEP6缺乏在体内的代谢后果。
通过使用公共转录组数据检查脂肪组织中Reep6的表达水平,并通过Western印迹和qPCR分析进行验证。在C3H10T1/2脂肪细胞和从REEP6KO小鼠获得的原代脂肪细胞中体外研究了REEP6的脂肪细胞特异性调节作用。通过间接量热法测量体内REEP6缺乏对能量消耗的影响。通过免疫印迹获取脂肪组织中的线粒体含量,线粒体DNA分析,以及共聚焦和电子显微镜。通过葡萄糖耐量试验在高脂饮食诱导的肥胖小鼠模型中测试REEP6KO对肥胖诱导的代谢功能障碍的影响。蛋白质印迹,和组织学分析。
REEP6表达在鼠脂肪细胞中高度富集,并且在脂肪细胞分化和冷暴露时急剧上调。REEP6在小鼠中的失活增加了肥胖,减少能量消耗和耐寒性。REEP6KO严重降低了BAT中蛋白激酶A介导的信号传导,并大大降低了线粒体质量。在培养的脂肪细胞中再现了REEP6失活对β-肾上腺素能信号传导减弱的影响,表明这种效应是细胞自主的。REEP6KO还抑制了棕色脂肪组织中腺苷酸环化酶3(Adcy3)的表达,并且脂肪细胞中REEP6的敲低降低了ADCY3对质膜的靶向。最后,REEP6KO加剧了高脂饮食诱导的胰岛素抵抗和脂肪组织炎症。
这项研究表明,REEP6在脂肪细胞中的β-肾上腺素能信号转导中起重要作用,涉及Adcy3的表达和运输。REEP6的基因失活减少了能量消耗,增加肥胖,以及肥胖相关代谢功能障碍的易感性。