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Abstract：
The interaction between Eu(III) ion and different pH buffers, popular in biology and biochemistry, viz. HEPES, PIPES, MES, MOPS, and TRIS, has been studied by solution nuclear magnetic resonance spectroscopy (NMR) and time-resolved laser-induced fluorescence spectroscopy (TRLFS) techniques. The Good\'s buffers reveal non-negligible interaction with Eu(III) as determined from their complex stability constants, where the sites of interaction are the morpholine and piperazine nitrogen atoms, respectively. In contrast, TRIS buffer shows practically no affinity towards Eu(III). Therefore, when investigating lanthanides, TRIS buffer should be preferred over Good\'s buffers.
摘要：
Eu(III)离子与不同pH值缓冲液的相互作用,在生物学和生物化学领域很受欢迎，viz.HEPES,管道,MES,MOPS,和TRIS,已通过溶液核磁共振波谱（NMR）和时间分辨激光诱导荧光光谱（TRLFS）技术进行了研究。Good\'s缓冲液显示出与Eu（III）的不可忽略的相互作用，这是根据其复杂的稳定常数确定的，其中相互作用的位点是吗啉和哌嗪氮原子，分别。相比之下,TRIS缓冲液对Eu(III)几乎没有亲和力。因此,当研究镧系元素时，TRIS缓冲区应优先于良好的缓冲区。
