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Abstract：
OBJECTIVE: Adipose tissue is a specialized endocrine organ that is involved in modulating whole-body energy homeostasis and expresses a specific subset of genes, which may play a role in adipose tissue metabolism. The aim of this study was to search for novel adipose tissue-specific genes using a tissue panel of RNAseq expression profiles.
METHODS: RNAseq expression profiles from 53 human tissues were downloaded from the GTex database. SLC19A3 expression was analyzed by microarray or real-time PCR in two sets of paired subcutaneous and omental adipose tissue samples, in two studies with adipose tissue from persons with high or low body mass index (BMI), in adipose tissue from patients who underwent weight loss with a very-low caloric diet and during preadipocyte-adipocyte differentiation.
RESULTS: The RNAseq-based tissue distribution expression screen identified SLC19A3 (encoding the thiamine transporter 2) as adipose tissue-specific. SLC19A3 expression was higher in subcutaneous compared with omental adipose tissue in both sample sets (p = 0.043 and p < 0.001). Preadipocyte differentiation towards adipocytes resulted in increased SLC19A3 gene expression (p = 0.018 or less at all-time points). Subcutaneous adipose tissue expression of SLC19A3 was lower in persons with high BMI in both cohorts (p = 0.008, and p < 0.001) and increased during a weight-loss intervention (p = 0.006).
CONCLUSIONS: The specific adipose tissue expression pattern of SLC19A3, together with its regulation in obesity and during weight loss, indicate that it plays a key role in adipocyte metabolism.
METHODS: RNAseq expression profiles from 53 human tissues were downloaded from the GTex database. SLC19A3 expression was analyzed by microarray or real-time PCR in two sets of paired subcutaneous and omental adipose tissue samples, in two studies with adipose tissue from persons with high or low body mass index (BMI), in adipose tissue from patients who underwent weight loss with a very-low caloric diet and during preadipocyte-adipocyte differentiation.
RESULTS: The RNAseq-based tissue distribution expression screen identified SLC19A3 (encoding the thiamine transporter 2) as adipose tissue-specific. SLC19A3 expression was higher in subcutaneous compared with omental adipose tissue in both sample sets (p = 0.043 and p < 0.001). Preadipocyte differentiation towards adipocytes resulted in increased SLC19A3 gene expression (p = 0.018 or less at all-time points). Subcutaneous adipose tissue expression of SLC19A3 was lower in persons with high BMI in both cohorts (p = 0.008, and p < 0.001) and increased during a weight-loss intervention (p = 0.006).
CONCLUSIONS: The specific adipose tissue expression pattern of SLC19A3, together with its regulation in obesity and during weight loss, indicate that it plays a key role in adipocyte metabolism.
摘要：
目的：脂肪组织是一种专门的内分泌器官，参与调节全身能量稳态并表达特定的基因子集，可能在脂肪组织代谢中起作用。这项研究的目的是使用RNAseq表达谱的组织组搜索新的脂肪组织特异性基因。
方法:从GTex数据库下载来自53个人组织的RNAseq表达谱。通过微阵列或实时PCR分析两组配对的皮下和网膜脂肪组织样品中的SLC19A3表达，在两项高或低体重指数（BMI）人群的脂肪组织研究中，在极低热量饮食和前脂肪细胞-脂肪细胞分化过程中体重减轻的患者的脂肪组织中。
结果：基于RNAseq的组织分布表达筛选将SLC19A3（编码硫胺素转运蛋白2）鉴定为脂肪组织特异性。在两个样品组中,SLC19A3在皮下的表达高于网膜脂肪组织(p=0.043和p<0.001)。前脂肪细胞向脂肪细胞的分化导致SLC19A3基因表达增加(在所有时间点p=0.018或更小)。SLC19A3的皮下脂肪组织表达在两个队列中具有高BMI的人中较低(p=0.008,p<0.001),并且在减重干预期间增加(p=0.006)。
结论：SLC19A3的特定脂肪组织表达模式及其在肥胖和体重减轻过程中的调节，表明它在脂肪细胞代谢中起着关键作用。
方法:从GTex数据库下载来自53个人组织的RNAseq表达谱。通过微阵列或实时PCR分析两组配对的皮下和网膜脂肪组织样品中的SLC19A3表达，在两项高或低体重指数（BMI）人群的脂肪组织研究中，在极低热量饮食和前脂肪细胞-脂肪细胞分化过程中体重减轻的患者的脂肪组织中。
结果：基于RNAseq的组织分布表达筛选将SLC19A3（编码硫胺素转运蛋白2）鉴定为脂肪组织特异性。在两个样品组中,SLC19A3在皮下的表达高于网膜脂肪组织(p=0.043和p<0.001)。前脂肪细胞向脂肪细胞的分化导致SLC19A3基因表达增加(在所有时间点p=0.018或更小)。SLC19A3的皮下脂肪组织表达在两个队列中具有高BMI的人中较低(p=0.008,p<0.001),并且在减重干预期间增加(p=0.006)。
结论：SLC19A3的特定脂肪组织表达模式及其在肥胖和体重减轻过程中的调节，表明它在脂肪细胞代谢中起着关键作用。
