Abstract：
Etoposide (Eto) is an anti-cancer drug that is associated with serious adverse effects on male reproductive function. Omega-3 polyunsaturated fatty acids (ω-3 PUFAs) and selenium (Se) are known as anti-inflammatory, anti-apoptotic and anti-oxidant agents. This work was designed to investigate changes in the biochemical parameters as well as alterations in Sertoli cell vimentin expression, ultrastructure and ectoplasmic specializations (ESs) following Eto treatment and to assess the ameliorative effect of ω-3 versus Se on these alterations. Eighty four adult male albino rats were used and classified into four groups: group I (control group), group II (Eto group) received Eto in a single intra-peritoneal (IP) dose (60 mg/kg B.W.), group III (Eto & ω-3 group) received the single IP dose of Eto as well as ω-3 (300 mg/kg B.W./day by intra-gastric intubation) starting 5 days before Eto injection till the time of sacrifice & group IV (Eto & Se group) received the single IP dose of Eto as well as Se (0.5 mg/kg B.W./day IP) starting 5 days before Eto injection till the time of sacrifice. The rats were subdivided into 2 subgroups (a) and (b) that were sacrificed 3 and 7 days after Eto injection respectively. Eto administration in group II induced increase in malondialdehyde (MDA), decrease in superoxide dismutase (SOD), collapse of Sertoli cell vimentin filaments and ultrastructural degenerative changes in both Sertoli cells and ESs. Se (group IV) reversed Eto toxic effects potently, while ω-3 (group III) had some limited protective effects.
摘要：
依托泊苷（Eto）是一种抗癌药物，对男性生殖功能具有严重的不良反应。ω-3多不饱和脂肪酸（ω-3PUFA）和硒（Se）被称为抗炎，抗凋亡和抗氧化剂。这项工作旨在研究生化参数的变化以及支持细胞波形蛋白表达的变化。Eto治疗后的超微结构和外质专业化（ESs），并评估ω-3与Se对这些改变的改善作用。选用84只成年雄性白化大鼠,分为4组:I组(对照组),II组（Eto组）以单次腹膜内（IP）剂量（60mg/kgB.W.）接受Eto，第III组（Eto和ω-3组）在Eto注射前5天开始接受单次IP剂量的Eto和ω-3（通过胃内插管进行300mg/kgB.W./天），直到处死和第IV组（Eto和Se组）在Eto注射前5天开始接受单次IP剂量的Eto和Se（0.5mg/kgB.W./天IP）。将大鼠再分为2个亚组(a)和(b),分别在Eto注射后3天和7天处死。II组的Eto给药诱导丙二醛（MDA）增加，超氧化物歧化酶（SOD）降低，支持细胞波形蛋白丝的塌陷和支持细胞和ESs的超微结构变性变化。硒（IV组）有效逆转了埃托毒性作用，而ω-3（组III）的保护作用有限。
