PDF(Pubmed)
Abstract：
The aim of this study was to evaluate the effect of Hermetia illucens larvae meal (HI) on the growth performance and intestinal barrier function of weaned pigs. To achieve this, 72 weaned pigs [28-day-old, 8.44 ± 0.04 kg body weight (BW)] were randomly assigned to three dietary treatments: basal diet (negative control, NC), zinc oxide-supplemented diet (positive control, PC), and HI-supplemented diet [100% replacement of fishmeal (FM), HI], for 28 days in the presence of enterotoxigenic Escherichia coli (ETEC). The results showed that HI and PC increased (p < 0.05) the average daily gain (ADG) and average daily feed intake (ADFI) of weaned pigs from day 1 to 14, and decreased diarrhea incidence from day 1 to 28. Additionally, HI increased (p < 0.05) claudin-1, occludin, mucin-1 (MUC-1), and MUC-2 expression, goblet cell number, and secretory immunoglobulin A (sIgA) concentration in the intestine of weaned pigs. Compared with NC, HI downregulated (p < 0.05) interleukin-1β (IL-1β) and IL-8 expression, and upregulated IL-10, transforming growth factor-β (TGF-β), antimicrobial peptide [porcine β defensin 1 (pBD1), pBD2, protegrin 1-5 (PG1-5)] expression in the jejunum or ileum. Moreover, HI decreased (p < 0.05) toll-like receptor 2 (TLR2), phosphorylated nuclear factor-κB (p-NF-κB), and phosphorylated mitogen-activated protein kinase (p-MAPK) expression, and increased sirtuin 1 (SIRT1) expression in the ileum. Additionally, HI increased histone deacetylase 3 (HDAC3) expression and acetylation of histone 3 lysine 27 (acH3k27) in the ileum. Furthermore, HI positively influenced the intestinal microbiota composition and diversity of weaned pigs and increased (p < 0.05) butyrate and valerate concentrations. Overall, dietary HI improved growth performance and intestinal barrier function, as well as regulated histone acetylation and TLR2-NF-κB/MAPK signaling pathways in weaned pigs.
摘要：
本研究旨在评价虫草幼虫粉(HI)对断奶仔猪生长性能和肠道屏障功能的影响。为了实现这一点，72头断奶猪[28日龄，8.44±0.04kg体重（BW）]被随机分配到三种饮食处理：基础饮食（阴性对照，NC),补充氧化锌的饮食（阳性对照，PC),和补充HI的饮食[100%替代鱼粉(FM)，HI],在存在产肠毒素大肠杆菌（ETEC）的情况下持续28天。结果表明，从第1天到第14天，HI和PC增加了断奶猪的平均日增重（ADG）和平均日采食量（ADFI），从第1天到第28天降低了腹泻发生率（p<0.05）。此外,HI增加（p<0.05）claudin-1，occludin，粘蛋白-1（MUC-1），和MUC-2表达，杯状细胞号,断奶仔猪肠道分泌型免疫球蛋白A(sIgA)浓度。与NC相比,HI下调（p<0.05）白细胞介素-1β（IL-1β）和IL-8表达，并上调IL-10，转化生长因子-β（TGF-β），抗菌肽[猪β防御素1（pBD1），pBD2，蛋白1-5（PG1-5）]在空肠或回肠中的表达。此外，HI降低（p<0.05）Toll样受体2（TLR2），磷酸化核因子-κB（p-NF-κB），和磷酸化丝裂原活化蛋白激酶（p-MAPK）表达，回肠中沉默调节蛋白1（SIRT1）的表达增加。此外,HI增加了回肠中组蛋白脱乙酰酶3（HDAC3）的表达和组蛋白3赖氨酸27（acH3k27）的乙酰化。此外，HI对断奶猪的肠道菌群组成和多样性有正向影响，并增加了丁酸和戊酸的浓度（p<0.05）。总的来说,日粮HI改善生长性能和肠道屏障功能,以及调节断奶猪的组蛋白乙酰化和TLR2-NF-κB/MAPK信号通路。
