METHODS: Two-hundred and eight prostate cancer patients who underwent tumor resection were continuously reviewed in this study. Tumor tissue and paired adjacent tissue were obtained and polymerase chain reaction assay was performed to detect the expression of lncRNA TUG1 in tumor tissue. PANC-1 cells were obtained and transferred with blank mimic, lncRNA TUG1 mimic, blank inhibitor, and lncRNA TUG1 inhibitor plasmids, then cell proliferation was detected by CCK-8, and cell apoptosis was evaluated by AV/PI assay.
RESULTS: LncRNA TUG1 expression was elevated in tumor tissue compared with paired adjacent tissue (P<0.001). Moreover, lncRNA TUG1 expression in tumor tissue positively correlated with pathological T stage (P=0.009) and lymph node metastasis (P=0.040). Patients with a high expression of lncRNA TUG1 had both worse disease-free survival (DFS) (P<0.001) and overall survival (OS) (P=0.003) compared with patients with a low expression of lncRNA TUG1. Multivariate Cox\'s proportional hazards regression analysis revealed that high lncRNA TUG1 expression was an independent predicting factor for both worse DFS (P=0.001) and OS (P=0.003). Moreover, CCK-8 and AV/PI assays demonstrated that lncRNA TUG1 enhanced cell proliferation and repressed cell apoptosis in PANC-1 cells.
CONCLUSIONS: A high expression of LncRNA TUG1 in tumor tissue correlates with progressive tumor condition and poorer survival in prostate cancer patients, and promotes proliferation while inhibiting cell apoptosis in prostate cancer cells.
方法:本研究连续回顾了200名接受肿瘤切除的前列腺癌患者。获得肿瘤组织和配对的癌旁组织,进行聚合酶链反应实验以检测lncRNATUG1在肿瘤组织中的表达。获得PANC-1细胞并用空白模拟物转移,lncRNATUG1模拟物,空白抑制剂,和lncRNATUG1抑制剂质粒,然后用CCK-8检测细胞增殖,用AV/PI法评价细胞凋亡。
结果:LncRNATUG1在肿瘤组织中的表达高于配对的邻近组织(P<0.001)。此外,lncRNATUG1在肿瘤组织中的表达与病理T分期(P=0.009)和淋巴结转移(P=0.040)呈正相关。与lncRNATUG1低表达患者相比,lncRNATUG1高表达患者的无病生存期(DFS)(P<0.001)和总生存期(OS)(P=0.003)均较差。多因素Cox比例风险回归分析显示,高lncRNATUG1表达是DFS(P=0.001)和OS(P=0.003)恶化的独立预测因素。此外,CCK-8和AV/PI测定表明,lncRNATUG1增强了PANC-1细胞的细胞增殖并抑制了细胞凋亡。
结论:肿瘤组织中LncRNATUG1的高表达与前列腺癌患者的进展性肿瘤状况和较差的生存率相关,并促进前列腺癌细胞增殖,同时抑制细胞凋亡。