Abstract：
Although anaerobic bacteria exist in abundance in cystic fibrosis (CF) airways, their role in disease progression is poorly understood. We hypothesized that the presence and relative abundance of the most prevalent, live, anaerobic bacteria in sputum of adults with CF were associated with adverse clinical outcomes. This is the first study to prospectively investigate viable anaerobic bacteria present in the sputum microbiota and their relationship with long-term outcomes in adults with CF. We performed 16S rRNA analysis using a viability quantitative PCR technique on sputum samples obtained from a prospective cohort of 70 adults with CF and collected clinical data over an 8 year follow-up period. We examined the associations of the ten most abundant obligate anaerobic bacteria present in the sputum with annual rate of FEV1 change. The presence of Porphyromonas pasteri and Prevotella nanceiensis were associated with a greater annual rate of FEV1 change; -52.3 ml yr-1 (95 % CI-87.7;-16.9), -67.9 ml yr-1 (95 % CI-115.6;-20.1), respectively. Similarly, the relative abundance of these live organisms were associated with a greater annual rate of FEV1 decline of -3.7 ml yr-1 (95 % CI: -6.1 to -1.3, P=0.003) and -5.3 ml yr-1 (95 % CI: -8.7 to -1.9, P=0.002) for each log2 increment of abundance, respectively. The presence and relative abundance of certain anaerobes in the sputum of adults with CF are associated with a greater rate of long-term lung function decline. The pathogenicity of anaerobic bacteria in the CF airways should be confirmed with further longitudinal prospective studies with a larger cohort of participants.
摘要：
尽管厌氧菌在囊性纤维化（CF）气道中大量存在，它们在疾病进展中的作用知之甚少.我们假设最普遍的，活,成人CF患者痰中的厌氧菌与不良临床结局相关.这是第一项前瞻性研究存在于痰菌群中的活厌氧菌及其与成人CF患者长期结局的关系的研究。我们使用活力定量PCR技术对从70名CF成年人的前瞻性队列中获得的痰样本进行了16SrRNA分析，并在8年的随访期内收集了临床数据。我们检查了痰中存在的十种最丰富的专性厌氧菌与FEV1年变化率的关联。pasteri和nanceiensisPrevotella的存在与FEV1的年变化率更大有关；-52.3mlyr-1（95％CI-87.7；-16.9），-67.9mlyr-1（95%CI-115.6；-20.1），分别。同样，这些活生物体的相对丰度与FEV1的年下降率相关，为-3.7mlyr-1（95％CI：-6.1至-1.3，P=0.003）和-5.3mlyr-1（95％CI：-8.7至-1.9，P=0.002），分别。成人CF患者痰中某些厌氧菌的存在和相对丰度与长期肺功能下降的更高比率相关。CF气道厌氧菌的致病性应该通过更多参与者的纵向前瞻性研究来证实。
