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Abstract：
To characterize the cold tolerance mechanism of the Pacific white shrimp (Litopenaeus vannamei), we performed single-cell RNA sequencing (scRNA-seq) of ∼5185 hepatopancreas cells from cold-tolerant (Lv-T) and common (Lv-C) L. vannamei at preferred and low temperatures (28°C and 10°C, respectively). The cells fell into 10 clusters and 4 cell types: embryonic, resorptive, blister-like, and fibrillar. We identified differentially expressed genes between Lv-T and Lv-C, which were mainly associated with the terms \"immune system,\" \"cytoskeleton,\" \"antioxidant system,\" \"digestive enzyme,\" and \"detoxification,\" as well as the pathways \"metabolic pathways of oxidative phosphorylation,\" \"metabolism of xenobiotics by cytochrome P450,\" \"chemical carcinogenesis,\" \"drug metabolism-cytochrome P450,\" and \"fatty acid metabolism.\" Reconstruction of fibrillar cell trajectories showed that, under low temperature stress, hepatopancreas cells had two distinct fates, cell fate 1 and cell fate 2. Cell fate 1 was mainly involved in signal transduction and sensory organ development. Cell fate 2 was mainly involved in metabolic processes. This study preliminarily clarifies the molecular mechanisms underlying cold tolerance in L. vannamei, which will be useful for the breeding of shrimp with greater cold tolerance.
摘要：
为了表征太平洋白虾（凡纳滨对虾）的耐寒机制，我们在首选和低温（28°C和10°C，分别)。细胞分为10个簇和4种细胞类型：胚胎，再吸收，像水泡一样，和纤丝。我们确定了Lv-T和Lv-C之间的差异表达基因，这主要与术语“免疫系统”有关，\"\"细胞骨架,“\”抗氧化系统，“消化酶”，“和”排毒，以及氧化磷酸化的代谢途径，“\”细胞色素P450的异源生物代谢，“\”化学致癌作用，“药物代谢-细胞色素P450”和“脂肪酸代谢”。“纤维状细胞轨迹的重建表明，在低温胁迫下，肝胰腺细胞有两种不同的命运，细胞命运1和细胞命运2。细胞命运1主要参与信号转导和感觉器官发育。细胞命运2主要参与代谢过程。本研究初步阐明了凡纳滨对虾耐寒的分子机制。这将有助于抗寒性更强的虾的育种。
