Abstract：
Treacle ribosome biogenesis factor 1 (TCOF1) plays a crucial role in multiple processes, including ribosome biogenesis, DNA damage response (DDR), mitotic regulation, and telomere integrity. However, its role in cancers remains unclear. We aimed to visualize the expression, prognostic, and mutational landscapes of TCOF1 across cancers and to explore its association with immune infiltration. In this work, we integrated information from TCGA and GEO to explore the differential expression and prognostic value of TCOF1. Then, the mutational profiles of TCOF1 in cancers were investigated. We further determined the correlation between TCOF1 and immune cell infiltration levels. Additionally, we determined correlations among certain immune checkpoints, microsatellite instability, tumor mutational burden (TMB), and TCOF1. Potential pathways of TCOF1 in tumorigenesis were analyzed as well. In general, tumor tissue had a higher expression level of TCOF1 than normal tissue. The prognostic value of TCOF1 was multifaceted, depending on type of cancer. TCOF1 was correlated with tumor purity, CD8+ T cells, CD4+ T cells, B cells, neutrophils, macrophages, and dendritic cells (DCs) in 6, 14, 16, 12, 20, 13, and 17 cancer types, respectively. TCOF1 might act on ATPase activity, microtubule binding, tubulin binding, and catalytic activity (on DNA), and participate in tumorigenesis through \"cell cycle\" and \"cellular-senescence\" pathways. TCOF1 could affect pan-cancer prognosis and was correlated with immune cell infiltration. \"Cell cycle\" and \"cellular-senescence\" pathways were involved in the functional mechanisms of TCOF1, a finding that awaits further experimental validation.
摘要：
反应核糖体生物发生因子1(TCOF1)在多个过程中起着至关重要的作用,包括核糖体生物发生，DNA损伤反应(DDR)有丝分裂调节，端粒完整性。然而,其在癌症中的作用尚不清楚。我们的目标是可视化表情，预后，和TCOF1在癌症中的突变景观，并探讨其与免疫浸润的关系。在这项工作中,我们整合了TCGA和GEO的信息，以探讨TCOF1的差异表达和预后价值。然后，研究了TCOF1在癌症中的突变谱.我们进一步确定了TCOF1与免疫细胞浸润水平之间的相关性。此外,我们确定了某些免疫检查点之间的相关性，微卫星不稳定，肿瘤突变负荷（TMB），和TCOF1。还分析了TCOF1在肿瘤发生中的潜在途径。总的来说,肿瘤组织中TCOF1的表达水平高于正常组织。TCOF1的预后价值是多方面的，取决于癌症的类型。TCOF1与肿瘤纯度相关，CD8+T细胞，CD4+T细胞,B细胞,中性粒细胞，巨噬细胞，和6、14、16、12、20、13和17种癌症类型的树突状细胞（DC），分别。TCOF1可能作用于ATP酶活性，微管结合，微管蛋白结合，和催化活性（对DNA），并通过“细胞周期”和“细胞衰老”途径参与肿瘤发生。TCOF1可以影响泛癌症的预后，并与免疫细胞浸润有关。“细胞周期”和“细胞衰老”途径参与了TCOF1的功能机制，这一发现有待进一步的实验验证。
