Abstract：
As a food adapted to all kinds of people, milk has a high nutritional value. Because milk is a complex biological matrix, detecting illegal compounds is often difficult. As a common pesticide, organophosphorus (OP) residues caused by nonstandard use may be ignored, which is a threat to milk quality. In this study, using coumaphos as template molecule, the synthesized molecularly imprinted polymer (MIP) can specifically recognize 7 kinds of OP. Then, the MIP was used as an identification element to prepare a chemiluminescence sensor on a 96-well microplate for the determination of OP residues in milk samples. Due to the 4-(imidazol-1-yl)phenol-enhanced luminol-H2O2 system, the sensitivity of the system is very high; the detection limits of 7 OP including coumaphos, fenthion, chlorpyrifos, parathion, diazinon, fenchlorphos, and fenitrothion were 1 to 3 pg/mL, and the half maximal inhibitory concentrations were 1 to 20 ng/mL. The intraday recoveries of 7 OP were in the range of 86.1 to 86.5%, and the interday recoveries were in the range of 83.6 to 94.2%. Furthermore, the sensor can be reused up to 5 times. Therefore, the MIP-based chemiluminescence sensor can be used as a routine tool to detect OP residues in milk samples.
摘要：
作为一种适应各种人群的食物，牛奶具有很高的营养价值。因为牛奶是一种复杂的生物基质，检测非法化合物通常很困难。作为一种常见的杀虫剂,不规范使用引起的有机磷（OP）残留可以忽略，这对牛奶质量构成威胁。在这项研究中,使用库马福斯作为模板分子，合成的分子印迹聚合物(MIP)可以特异性识别7种OP。然后，MIP被用作识别元件，在96孔微孔板上制备化学发光传感器，用于测定牛奶样品中的OP残留。由于4-（咪唑-1-基）苯酚增强的鲁米诺-H2O2系统，该系统的灵敏度非常高；检测限7OP，包括库马福司，fenthion,毒死蜱,对硫磷,二嗪农,fenchlophos,和非硝硫磷为1至3pg/mL，半最大抑制浓度为1~20ng/mL。7OP的日内回收率在86.1％至86.5％之间，日间回收率在83.6至94.2%之间。此外，传感器可以重复使用多达5次。因此,基于MIP的化学发光传感器可以用作检测牛奶样品中OP残留的常规工具。
