Abstract：
OBJECTIVE: The aim of this study was to investigate the presence of synovial mast cells (MCs) in hip and knee tissue from osteoarthritis (OA) patients and to correlate them with clinical and radiological data.
METHODS: Synovial tissue was obtained during arthroplasty from 60 patients, 30 with knee OA and 30 with hip OA. Control synovial tissue was obtained from 30 patients without OA, 15 undergoing above-knee amputation and 15 receiving a hip replacement for fracture. Before surgery, the radiographic findings were graded according to the Kellgren-Lawrence system and clinical data including pain (VAS) and functional information (KOOS and HOOS) was collected. The tissue was stained with hematoxylin-eosin and toluidine blue for histochemistry and incubated with CD117 and CD31 antibodies for immunohistochemistry. MC and vessel number and synovitis score were determined in all samples.
RESULTS: Mean MC number, synovitis score and vessel number were significantly higher in the OA samples (p < 0.05) than in control tissue. MC number correlated with the synovitis score and disease severity in both patient groups.
CONCLUSIONS: The prevalence of MCs in synovium from OA patients and their association with synovial inflammation and pain suggest a role for them in OA pathophysiology.
METHODS: Synovial tissue was obtained during arthroplasty from 60 patients, 30 with knee OA and 30 with hip OA. Control synovial tissue was obtained from 30 patients without OA, 15 undergoing above-knee amputation and 15 receiving a hip replacement for fracture. Before surgery, the radiographic findings were graded according to the Kellgren-Lawrence system and clinical data including pain (VAS) and functional information (KOOS and HOOS) was collected. The tissue was stained with hematoxylin-eosin and toluidine blue for histochemistry and incubated with CD117 and CD31 antibodies for immunohistochemistry. MC and vessel number and synovitis score were determined in all samples.
RESULTS: Mean MC number, synovitis score and vessel number were significantly higher in the OA samples (p < 0.05) than in control tissue. MC number correlated with the synovitis score and disease severity in both patient groups.
CONCLUSIONS: The prevalence of MCs in synovium from OA patients and their association with synovial inflammation and pain suggest a role for them in OA pathophysiology.
摘要：
目的：本研究的目的是研究骨关节炎（OA）患者的髋关节和膝关节组织中滑膜肥大细胞（MC）的存在，并将其与临床和放射学数据相关联。
方法：在关节成形术中获得了60例患者的滑膜组织，30与膝盖OA和30与髋关节OA。对照滑膜组织取自30例无OA患者,15例接受膝上截肢，15例接受骨折髋关节置换术。手术前,根据Kellgren-Lawrence系统对影像学检查结果进行分级,并收集包括疼痛(VAS)和功能信息(KOOS和HOOS)在内的临床数据.将组织用苏木精-伊红和甲苯胺蓝染色以进行组织化学，并与CD117和CD31抗体一起孵育以进行免疫组织化学。在所有样品中测定MC和血管数目和滑膜炎评分。
结果：平均MC数，OA样本中的滑膜炎评分和血管数目显著高于对照组织(p<0.05)。两组患者的MC数与滑膜炎评分和疾病严重程度相关。
结论：OA患者滑膜中MC的患病率及其与滑膜炎症和疼痛的关系表明它们在OA病理生理学中的作用。
方法：在关节成形术中获得了60例患者的滑膜组织，30与膝盖OA和30与髋关节OA。对照滑膜组织取自30例无OA患者,15例接受膝上截肢，15例接受骨折髋关节置换术。手术前,根据Kellgren-Lawrence系统对影像学检查结果进行分级,并收集包括疼痛(VAS)和功能信息(KOOS和HOOS)在内的临床数据.将组织用苏木精-伊红和甲苯胺蓝染色以进行组织化学，并与CD117和CD31抗体一起孵育以进行免疫组织化学。在所有样品中测定MC和血管数目和滑膜炎评分。
结果：平均MC数，OA样本中的滑膜炎评分和血管数目显著高于对照组织(p<0.05)。两组患者的MC数与滑膜炎评分和疾病严重程度相关。
结论：OA患者滑膜中MC的患病率及其与滑膜炎症和疼痛的关系表明它们在OA病理生理学中的作用。
