Abstract：
The IPN virus (IPNV) causes a highly contagious disease that affects farmed salmonids. IPNV isolates have been phylogenetically classified into seven genogroups, of which two are present in Chile, genogroups 1 and 5. This study aimed to compare the transcriptomic response of rainbow trout fry challenged with two Chilean isolates of IPNV, RTTX (genogroup 1), and ALKA (genogroup 5). Tissue samples from challenged individuals and controls were taken at 1, 7, and 20 days post-challenge and analyzed by RNA-Seq. The results revealed that infection with RTTX elicited a greater modulation of the trout transcriptome compared to ALKA infection, generating a greater number of highly differentially expressed genes in relation to the control fish. Gene Ontology enrichment indicated that functions related to the inflammatory and immune responses were modulated in fish challenged with both isolates throughout the trial, but with different regulation patterns. On day 1 post challenge, these functions were activated in those challenged with ALKA, but suppressed in RTTX-challenged fish. These results suggest that rainbow trout exhibit a differential transcriptomic response to infection with the two genetically distinct IPNV isolates, especially at early times post-infection.
摘要：
IPN病毒（IPNV）引起高度传染性疾病，影响养殖的鲑鱼。IPNV分离株已被系统发育分类为七个基因组，其中两个在智利，基因组1和5。本研究旨在比较两种智利IPNV分离株对虹鳟鱼鱼苗的转录组反应，RTTX(基因组1)，和ALKA（基因组5）。在攻击后1、7和20天采集来自攻击个体和对照的组织样品,并通过RNA-Seq分析。结果显示，与ALKA感染相比，RTTX感染引起鳟鱼转录组的更大调节，与对照鱼相比，产生更多的高度差异表达基因。基因本体论富集表明，在整个试验过程中，与两种分离株攻击的鱼类中，与炎症和免疫反应相关的功能得到了调节，但是有不同的监管模式。在挑战后的第1天，这些功能在那些受到ALKA挑战的人中被激活，但在RTTX挑战的鱼中被抑制。这些结果表明，虹鳟鱼对两种遗传上不同的IPNV分离株的感染表现出差异的转录组反应，尤其是在感染后的早期。
