PDF(Pubmed)
Abstract：
DNA barcodes are regarded as hereditary succession codes that serve as a recognition marker to address several queries relating to the identification, classification, community ecology, and evolution of certain functional traits in organisms. The mitochondrial cytochrome c oxidase 1 (CO1) gene as a DNA barcode is highly efficient for discriminating vertebrate and invertebrate animal species. Similarly, different specific markers are used for other organisms, including ribulose bisphosphate carboxylase (rbcL), maturase kinase (matK), transfer RNA-H and photosystem II D1-ApbsArabidopsis thaliana (trnH-psbA), and internal transcribed spacer (ITS) for plant species; 16S ribosomal RNA (16S rRNA), elongation factor Tu gene (Tuf gene), and chaperonin for bacterial strains; and nuclear ITS for fungal strains. Nevertheless, the taxon coverage of reference sequences is far from complete for genus or species-level identification. Applying the next-generation sequencing approach to the parallel acquisition of DNA barcode sequences could greatly expand the potential for library preparation or accurate identification in biodiversity research. Overall, this review articulates on the DNA barcoding technology as applied to different organisms, its universality, applicability, and innovative approach to handling DNA-based species identification.
摘要：
DNA条形码被认为是遗传继承代码，可作为识别标记来解决与识别有关的几个问题。分类，群落生态学,以及生物体某些功能性状的进化。线粒体细胞色素c氧化酶1（CO1）基因作为DNA条形码对于区分脊椎动物和无脊椎动物物种非常有效。同样，不同的特定标记用于其他生物，包括核糖二磷酸羧化酶（rbcL），成熟酶激酶（matK），转移RNA-H和光系统IID1-Apbs拟南芥（trnH-psbA），和植物物种的内部转录间隔区(ITS)；16S核糖体RNA(16SrRNA)，延伸因子Tu基因（Tuf基因），细菌菌株和伴侣蛋白；真菌菌株的核ITS。然而，参考序列的分类单元覆盖对于属或物种水平的鉴定而言远未完成。将下一代测序方法应用于DNA条形码序列的平行获取可以极大地扩展在生物多样性研究中的文库制备或准确鉴定的潜力。总的来说,这篇综述阐述了应用于不同生物的DNA条形码技术，其普遍性,适用性,以及处理基于DNA的物种鉴定的创新方法。
