Abstract：
An early and accurate diagnosis of early onset neonatal sepsis (EONS) and late onset neonatal sepsis (LONS) is essential to improve the outcome of this devastating conditions. Especially, preterm infants are at risk. Reliable biomarkers are rare, clinical decision-making depends on clinical appearance and multiple laboratory findings. Markers of NET formation and NET turnover might improve diagnostic precision. Aim of this study was to evaluate the diagnostic value of NETs in sepsis diagnosis in neonatal preterm infants.
Plasma samples of neonatal preterm infants with suspected sepsis were collected. Blood samples were assayed for markers of NET formation and NET turnover: cfDNA, DNase1, nucleosome, NE, and H3Cit. All clinical findings, values of laboratory markers, and epidemiological characteristics were collected retrospectively. Two subpopulations were created to divide EONS from LONS. EMA sepsis criteria for neonatal sepsis were used to generate a sepsis group (EMA positive) and a control group (EMA negative).
A total of 31 preterm neonates with suspected sepsis were included. Out of these, nine patients met the criteria for sepsis according to EMA. Regarding early onset neonatal sepsis (3 EONS vs. 10 controls), cfDNA, DNase I, nucleosome, and CRP were elevated significantly. H3Cit and NE did not show any significant elevations. In the late onset sepsis collective (6 LONS vs. 12 controls), cfDNA, DNase I, and CRP differed significantly compared to control group.
Plasma samples of neonatal preterm infants with suspected sepsis were collected. Blood samples were assayed for markers of NET formation and NET turnover: cfDNA, DNase1, nucleosome, NE, and H3Cit. All clinical findings, values of laboratory markers, and epidemiological characteristics were collected retrospectively. Two subpopulations were created to divide EONS from LONS. EMA sepsis criteria for neonatal sepsis were used to generate a sepsis group (EMA positive) and a control group (EMA negative).
A total of 31 preterm neonates with suspected sepsis were included. Out of these, nine patients met the criteria for sepsis according to EMA. Regarding early onset neonatal sepsis (3 EONS vs. 10 controls), cfDNA, DNase I, nucleosome, and CRP were elevated significantly. H3Cit and NE did not show any significant elevations. In the late onset sepsis collective (6 LONS vs. 12 controls), cfDNA, DNase I, and CRP differed significantly compared to control group.
摘要：
早期和准确诊断早发性新生儿败血症（EONS）和晚发性新生儿败血症（LONS）对于改善这种破坏性疾病的结局至关重要。尤其是,早产儿有风险。可靠的生物标志物很少见，临床决策取决于临床表现和多项实验室检查结果.网络形成和网络周转的标记可能会提高诊断精度。本研究的目的是评估NETs在新生儿早产儿败血症诊断中的诊断价值。
收集疑似败血症的新生儿早产儿的血浆样品。检测血样中网络形成和网络周转的标志物：cfDNA，DNase1，核小体，NE,H3Cit所有临床发现,实验室标记的值，回顾性收集流行病学特征。创建了两个亚群，以将EONS与LONS分开。使用新生儿败血症的EMA败血症标准来产生败血症组（EMA阳性）和对照组（EMA阴性）。
共纳入31例疑似败血症的早产儿。在这些中，9例患者符合EMA的脓毒症标准.关于早发性新生儿败血症（3EONSvs.10个控件），cfDNA,DNaseI,核小体,CRP明显升高。H3Cit和NE没有显示任何显著升高。在晚期败血症集体中（6LONSvs.12个控件)，cfDNA,DNaseI,与对照组相比，CRP有显著差异。
收集疑似败血症的新生儿早产儿的血浆样品。检测血样中网络形成和网络周转的标志物：cfDNA，DNase1，核小体，NE,H3Cit所有临床发现,实验室标记的值，回顾性收集流行病学特征。创建了两个亚群，以将EONS与LONS分开。使用新生儿败血症的EMA败血症标准来产生败血症组（EMA阳性）和对照组（EMA阴性）。
共纳入31例疑似败血症的早产儿。在这些中，9例患者符合EMA的脓毒症标准.关于早发性新生儿败血症（3EONSvs.10个控件），cfDNA,DNaseI,核小体,CRP明显升高。H3Cit和NE没有显示任何显著升高。在晚期败血症集体中（6LONSvs.12个控件)，cfDNA,DNaseI,与对照组相比，CRP有显著差异。
