Abstract：
Canine brucellosis, a worldwide zoonotic disease, is mainly caused by Brucella canis. In the present study, we isolated a Brucella strain (CD3) from a subclinically infected pet dog in Sichuan Province, Southwestern China. Classical biotyping methods and molecular biological tests (BCSP31 and BcSS PCR) proved that the strain belonged to B. canis. Furthermore, B. canis CD3 and another two B. canis strains (WJ5 and YA4), which were all isolated from pet dogs in Sichuan, were genotyped using multilocus sequence typing (MLST). Our results showed that the three B. canis strains were identified as the same sequence type (ST21). The present study is the first to report B. canis strain from a subclinically infected pet dog in China, indicating a potential threat to public health posed by subclinical infections in pet dogs. We suggest that screening for B. canis should be incorporated into routine medical examination of pet dogs and other companion animals in areas with a history of animal or human brucellosis.
摘要：
犬布鲁氏菌病，一种世界性的人畜共患疾病,主要是由犬布鲁氏菌引起的。在本研究中,我们从四川省一只亚临床感染的宠物狗中分离出一株布氏杆菌(CD3),中国西南部。经典生物分型方法和分子生物学试验(BCSP31和BcSSPCR)证明该菌株属于犬芽孢杆菌。此外，犬B.canisCD3和另外两个犬B.canis菌株（WJ5和YA4），它们都是从四川的宠物狗中分离出来的，使用多位点序列分型(MLST)进行基因分型。我们的结果表明，这三个犬芽孢杆菌菌株被鉴定为相同的序列类型（ST21）。本研究首次报道了中国亚临床感染宠物犬的犬只。表明宠物狗的亚临床感染对公众健康构成潜在威胁。我们建议在有动物或人类布鲁氏菌病病史的地区，对宠物狗和其他伴侣动物的常规医学检查中应纳入犬氏杆菌的筛查。
