PDF(Pubmed)
Abstract：
Bioactive collagen crosslinkers propose to render the dentin hybrid layer less perceptive to hydrolytic challenge. This study aims to evaluate whether bond strength of dental resin composite to dentin benefits from riboflavin (RB)-sensitized crosslinking when used in a clinically applicable protocol. A total of 300 human dentin specimens were prepared consistent with the requirements for a macro-shear bond test. RB was applied on dentin, either incorporated in the primer (RBp) of a two-step self-etch adhesive or as an aqueous solution (RBs) before applying the adhesive, and blue light from a commercial polymerization device was used for RB photoactivation. Bonding protocol executed according to the manufacturer\'s information served as control. Groups (n = 20) were tested after 1 week, 1 month, 3 months, 6 months or 1 year immersion times (37 °C, distilled water). The different application methods of RB significantly influenced bond strength (p < 0.001) with a medium impact (η2p = 0.119). After 1 year immersion, post hoc analysis identified a significant advantage for RB groups compared to RBp (p = 0.018), which is attributed to a pH-/solvent-dependent efficiency of RB-sensitized crosslinking, stressing the importance of formulation adjustments. We developed an application protocol for RB-sensitized crosslinking with emphasis on clinical applicability to test its performance against a gold-standard adhesive, and are confident that, with a few adjustments to the application solution, RB-sensitized crosslinking can improve the longevity of adhesive restorations in clinics.
摘要：
生物活性胶原交联剂提出使牙本质杂化层对水解挑战的感知较小。这项研究旨在评估牙科树脂复合材料与牙本质的粘结强度是否在临床适用的方案中使用核黄素（RB）敏化的交联中受益。根据宏观剪切粘结测试的要求制备总共300个人牙本质样本。RB应用于牙本质，在施加粘合剂之前，将两步自蚀粘合剂掺入底漆（RBp）或作为水溶液（RBs），并且来自商业聚合装置的蓝光用于RB光活化。根据制造商的信息执行的绑定协议作为控制。1周后测试组（n=20），1个月,3个月,6个月或1年浸泡时间(37°C，蒸馏水)。RB的不同应用方法显着影响粘结强度（p<0.001），中等冲击（η2p=0.119）。浸泡1年后，事后分析发现，与RBp相比，RB组有显著优势(p=0.018)，这归因于RB敏化交联的pH/溶剂依赖性效率，强调制定调整的重要性。我们开发了RB敏化交联的应用方案，重点是临床适用性，以测试其与金标准粘合剂的性能，并有信心，通过对应用程序解决方案的一些调整，RB敏化交联可以提高临床上粘合剂修复的寿命。
