Abstract：
Many factors affecting satellitism tests are unclear, and it is difficult to avoid misidentification, even if the medium is properly selected. We investigated the factors causing false-positive results for Haemophilus influenzae and false-negative results for Haemophilus parainfluenzae in the satellitism tests using Staphylococcus aureus as the source of nicotinamide adenine dinucleotide (NAD). H. influenzae (four reference strains and 47 clinical isolates), H. parainfluenzae (two reference strains and 67 clinical isolates), four different media, and two strains of S. aureus revived on two different media were used in this study. The type of medium used to revive S. aureus was the most common factor causing false-positive results for H. influenzae, followed by different strains of S. aureus and the type of medium used for the experiment. The production of false-negative results for H. parainfluenzae was only related to the medium used in the experiment. To improve the accuracy of the tests in routine laboratories, using S. aureus as the source of NAD, tryptic soy agar, and S. aureus (ATCC 25923) revived on nutrient agar should be adopted.
摘要：
影响卫星测试的许多因素尚不清楚，很难避免错误识别，即使介质选择得当。我们在使用金黄色葡萄球菌作为烟酰胺腺嘌呤二核苷酸（NAD）来源的卫星测试中，调查了导致流感嗜血杆菌假阳性结果和副流感嗜血杆菌假阴性结果的因素。流感嗜血杆菌（4个参考菌株和47个临床分离株），H.parainfluenzae（两个参考菌株和67个临床分离株），四种不同的媒介,在这项研究中使用了在两种不同培养基上复活的两株金黄色葡萄球菌。用于恢复金黄色葡萄球菌的培养基类型是导致流感嗜血杆菌假阳性结果的最常见因素，其次是不同的金黄色葡萄球菌菌株和用于实验的培养基类型。副流感嗜血杆菌假阴性结果的产生仅与实验中使用的培养基有关。为了提高常规实验室测试的准确性，使用金黄色葡萄球菌作为NAD的来源，胰蛋白酶大豆琼脂，应采用在营养琼脂上复活的金黄色葡萄球菌(ATCC25923)。
