Abstract：
Shifts in skin microbiome are considered to be involved in the pathogenesis of psoriasis. However, data on the microbial dysbiosis of nail psoriasis are scarce. In this study, we aim to investigate and characterize the nail bacterial and fungal microbiome in patients with psoriasis. Nail samples were collected prospectively from 36 subjects with nail psoriasis, 24 psoriatic subjects without nail involvement and 32 healthy controls. Amplicon sequencing was performed to evaluate the bacterial and fungal community compositions. Significant alterations in the bacterial microbiome were found in the nail samples of psoriatic patients. The unaffected nails in psoriatic patients were associated with higher bacterial diversity, and a higher relative abundance of Enhydrobacter, whereas nail psoriasis was correlated with a decreased relative abundance of Anaerococcus. Shifts in fungal community composition were reflected by a higher proportion of Malassezia in the unaffected nails of psoriatic patients and an increased proportion of Candida in psoriatic nails. Shifts in the nail microbiome in psoriasis suggest a potential role of microbes in the development of nail psoriasis. Future researches focusing on these microorganisms may help to explain the pathogenesis of psoriasis.
摘要：
皮肤微生物组的变化被认为与银屑病的发病机理有关。然而,关于指甲牛皮癣的微生物菌群失调的数据很少。在这项研究中,我们的目的是调查和表征牛皮癣患者的指甲细菌和真菌微生物组。前瞻性地从36名患有指甲牛皮癣的受试者中收集了指甲样本，24名无指甲受累的银屑病患者和32名健康对照。进行扩增子测序以评估细菌和真菌群落组成。在银屑病患者的指甲样本中发现了细菌微生物组的显着变化。牛皮癣患者未受影响的指甲与较高的细菌多样性有关，和相对丰度较高的Enhydrobacter,而指甲牛皮癣与缺氧球菌的相对丰度降低有关。真菌群落组成的变化反映在银屑病患者未受影响的指甲中马拉色菌的比例较高，而银屑病指甲中念珠菌的比例增加。牛皮癣中指甲微生物组的变化表明微生物在指甲牛皮癣发展中的潜在作用。未来对这些微生物的研究可能有助于解释银屑病的发病机制。
