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Abstract：
Infection is responsible for up to 60% of deaths in burn patients, and Pseudomonas aeruginosa (PA) has the highest mortality rate among all causes of bacteremia. These aggressive and virulent infections are often not detected until there is a positive blood culture for PA, meaning the patient already has bacteremia. An assay that rapidly detects PA while it is still present at low numbers within the burn wound could therefore be transformative, as it would allow for early and more effective treatment intervention. Pyoverdine, an Fe3+ chelating fluorescent molecule, is produced by PA to overcome the problem of limited iron availability and is essential for PA to cause acute infections. Here, we report the development and use of a simple qualitative pyoverdine assay for detection of PA in a clinically relevant in vivo burn wound model. In contrast with the 24 h quantitative culture approaches currently used, the pyoverdine assay is complete within 15 min. The assay has a PA detection limit of ∼106 colony forming units/(g of tissue) and detects the PA-specific biomarker pyoverdine within burn wounds before the pathogen disseminates within the body, evidence that the assay can detect lower, prebacteremic levels of PA within burn wounds.
摘要：
感染是导致烧伤患者死亡的60%的原因，铜绿假单胞菌（PA）在菌血症的所有原因中死亡率最高。这些侵袭性和毒性感染通常直到PA的血培养阳性才被检测到，意思是病人已经有菌血症了.因此，在烧伤伤口中仍然存在少量时快速检测PA的检测方法可能是革命性的，因为这将允许早期和更有效的治疗干预。Pyoverdine,Fe3+螯合荧光分子,由PA产生以克服铁可用性有限的问题,并且是PA引起急性感染所必需的。这里,我们报道了在临床相关的体内烧伤创伤模型中检测PA的简单定性pyoverdine测定法的开发和使用。与目前使用的24小时定量培养方法相反，pyoverdine测定在15分钟内完成。TheassayhasaPAdetectionlimitof~106coluntformingunits/(goforganization)anddetectsthePA-specificblogicalingpyoverdinewithinthebourcaldisspreateswithinthebody,该测定法可以检测到更低的证据，烧伤创面内PA的菌前水平。
