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Abstract：
Lateral flow immunoassay (LFIA) is one of the most widely used tools for analysis and field measurement and has the advantages of high efficiency, simple operation, portability, and low cost. Therefore, in this study, we designed a proof-of-concept of LFIA based on rhodium nanoparticles and investigated its improvement by further introducing the tetramethyl benzidine and H2O2 mixture as the substrate to trigger the color reaction. The proposed methods were qualitative research by the naked eye and quantitative measurement by a smartphone and software. Under the optimal condition, the detection of ferritin was successfully established with the limit of detection of 0.3 ng/mL. The lowest visually detectable amount was 0.05 ng/mL. To verify the performance of the RhNPs-LFIA, three spiked serum samples were tested, and the recovery rate increased from 88.9 to 129.9%, revealing that the proposed methods were applicable and practically reliable for testing serum samples. The developed RhNP-based LFIA is highly sensitive and convenient, which provides a promising technology for accurate, rapid, high sensitivity, and high screening detection of ferritin in clinical diagnosis.
摘要：
侧流免疫分析(LFIA)是目前应用最广泛的分析和现场测量工具之一,操作简单,便携性,和低成本。因此,在这项研究中，我们设计了基于铑纳米颗粒的LFIA概念验证，并通过进一步引入四甲基联苯胺和H2O2混合物作为底物以引发显色反应来研究其改进。提出的方法是通过肉眼进行定性研究，通过智能手机和软件进行定量测量。在最优条件下,成功建立了铁蛋白的检测方法,检出限为0.3ng/mL。最低视觉可检测量为0.05ng/mL。要验证RhNP-LFIA的性能，测试了三个加标血清样本，回收率从88.9％提高到129.9％，揭示了所提出的方法是适用的和实际可靠的测试血清样品。开发的基于RhNP的LFIA是高度敏感和方便，它提供了一种有前途的技术，快速,高灵敏度,和高筛查检测铁蛋白在临床诊断中的应用。
