PDF(Pubmed)
Abstract：
κ-Carrageenan is a plant polysaccharide derived from red seaweeds reported to possess potential medicinal and antioxidants activities. The present study aimed to identify the cryoprotective effects of κ-carrageenan on the quality of frozen-thawed canine semen. Twenty-eight ejaculates were collected and diluted in a Tris egg-yolk-free extender supplemented with various concentrations of κ-carrageenan (0.0%, 0.1%, 0.2%, 0.3%, and 0.5%). The addition of κ-carrageenan to the extender at a 0.2% concentration induced a significant increase in the total motility (TM) and the rapid progressive motility (RPM) of canine sperm. Among the experimental groups, the highest percentage of sperms with intact acrosomes was found in the 0.5% κ-carrageenan group (p < 0.05). Apoptosis levels were significantly lower in the 0.1% and 0.2% κ-carrageenan treatment. Moreover, sperm in the κ-carrageenan supplemented group showed a significantly higher expression of antiapoptotic (Bcl-2) and lower expression of NADPH oxidase (NOX5), spermine synthase (SMS), and spermine oxidase (SMOX) genes than those in the control group. In conclusion, the addition of κ-carrageenan to the freezing extender improved the overall efficiency of frozen-thawed dog spermatozoa.
摘要：
κ-角叉菜胶是一种源自红海藻的植物多糖，据报道具有潜在的药用和抗氧化剂活性。本研究旨在研究κ-角叉菜胶对冻融犬精液质量的冷冻保护作用。收集28种射精，并在添加了各种浓度的κ-角叉菜胶的无Tris蛋黄补充剂中稀释（0.0％，0.1%,0.2%，0.3%,和0.5%）。以0.2％的浓度向增量剂中添加κ-角叉菜胶会导致犬精子的总运动性（TM）和快速渐进运动性（RPM）显着增加。在实验组中，0.5%κ-角叉菜胶组精子顶体完整百分率最高(p<0.05)。在0.1%和0.2%κ-角叉菜胶处理中,细胞凋亡水平显著降低。此外，补充κ-角叉菜胶组的精子显示出抗凋亡（Bcl-2）的表达显着升高，而NADPH氧化酶（NOX5）的表达降低，精胺合成酶（SMS），和精胺氧化酶(SMOX)基因高于对照组。总之,在冷冻补充剂中添加κ-角叉菜胶可提高冻融狗精子的整体效率。
