Abstract：
We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with Mycobacterium chelonae, which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples.
摘要：
我们介绍了一名72岁的女性患者，其生物复合移植物急性破裂，由于主动脉夹层而更换主动脉瓣和升主动脉后21个月。术中活检的氧胺-罗丹明染色显示抗酸杆菌，但是经典的培养和分子方法无法识别任何生物。宏基因组分析显示感染分枝杆菌,这通过靶特异性qPCR证实。样本的复杂性需要定制的生物信息学管道，包括去除人类序列的清洁步骤，牛ad猪的起源。我们的研究强调了多重测试的重要性，以增加高度复杂样品中病原体鉴定的可能性。
