PDF(Pubmed)
Abstract：
Apigenin is one of the most studied flavonoids and is widely distributed in the plant kingdom. Apigenin exerts important antioxidant, antibacterial, antifungal, antitumor activities, and anti-inflammatory effects in neurological or cardiovascular disease. Chalcone isomerase A (chiA) is an important enzyme of the flavonoid biosynthesis pathway. In order to enhance the apigenin production, the petunia chi A gene was transformed for Astragalus trigonus. Bialaphos survived plants were screened by PCR, dot blot hybridization and RT-PCR analysis. Also, jasmonic acid, salicylic acid, chitosan and yeast extract were tested to evaluate their capacity to work as elicitors for apigenin. Results showed that yeast extract was the best elicitor for induction of apigenin with an increase of 3.458 and 3.9 fold of the control for calli and cell suspension culture, respectively. Transformed cell suspension showed high apigenin content with a 20.17 fold increase compared to the control and 6.88 fold more than the yeast extract treatment. While, transformed T1 calli derived expressing chiA gene produced apigenin 4.2 fold more than the yeast extract treatment. It can be concluded that the highest accumulation of apigenin was obtained with chiA transgenic cell suspension system and it can be utilized to enhancement apigenin production in Astragalus trigonus.
摘要：
芹菜素是研究最多的类黄酮之一，广泛分布于植物界。芹菜素发挥重要的抗氧化作用,抗菌，抗真菌药,抗肿瘤活性，和神经或心血管疾病的抗炎作用。查尔酮异构酶A（chiA）是类黄酮生物合成途径的重要酶。为了提高芹菜素的产量，矮牵牛A基因转化为黄芪。用PCR方法筛选比拉福活植株,斑点杂交和RT-PCR分析。此外，茉莉酸,水杨酸,对壳聚糖和酵母提取物进行了测试，以评估它们作为芹菜素诱导因子的能力。结果表明，酵母提取物是诱导芹菜素的最佳诱导子，愈伤组织和细胞悬浮培养比对照增加了3.458和3.9倍，分别。转化的细胞悬浮液显示出高芹菜素含量，与对照相比增加了20.17倍，与酵母提取物处理相比增加了6.88倍。同时,源自表达chiA基因的转化的T1愈伤组织产生的芹菜素比酵母提取物处理多4.2倍。可以得出结论，用chiA转基因细胞悬浮系统获得了最高的芹菜素积累，可以用来提高黄芪中芹菜素的产量。
