Abstract：
Electroacupuncture (EA) has been widely used for functional restoration after stroke. However, its role in post-stroke rehabilitation and the associated regulatory mechanisms remain poorly understood. In this study, we applied EA to the Zusanli (ST36) and Quchi (LI11) acupoints in rats with middle cerebral artery occlusion and reperfusion. We found that EA effectively increased the expression of brain-derived neurotrophic factor and its receptor tyrosine kinase B, synapsin-1, postsynaptic dense protein 95, and microtubule-associated protein 2 in the ischemic penumbra of rats with middle cerebral artery occlusion and reperfusion. Moreover, EA greatly reduced the expression of myelin-related inhibitors Nogo-A and NgR in the ischemic penumbra. Tyrosine kinase B inhibitor ANA-12 weakened the therapeutic effects of EA. These findings suggest that EA can improve neurological function after middle cerebral artery occlusion and reperfusion, possibly through regulating the activity of the brain-derived neurotrophic factor/tyrosine kinase B signal pathway. All procedures and experiments were approved by the Animal Research Committee of Shanghai University of Traditional Chinese Medicine, China (approval No. PZSHUTCM200110002) on January 10, 2020.
摘要：
电针（EA）已广泛用于中风后的功能恢复。然而,其在卒中后康复中的作用以及相关的调节机制仍然知之甚少.在这项研究中,我们将EA应用于大脑中动脉闭塞和再灌注大鼠的足三里（ST36）和曲奇（LI11）穴位。我们发现电针能有效增加脑源性神经营养因子及其受体酪氨酸激酶B的表达,突触素-1，突触后致密蛋白95和微管相关蛋白2在大脑中动脉闭塞和再灌注大鼠缺血半暗带中的作用。此外，EA大大降低了缺血半影中髓磷脂相关抑制剂Nogo-A和NgR的表达。酪氨酸激酶B抑制剂ANA-12削弱了EA的治疗作用。提示电针能改善大脑中动脉闭塞再灌注后的神经功能,可能通过调节脑源性神经营养因子/酪氨酸激酶B信号通路的活性。所有程序和实验均经上海中医药大学动物研究委员会批准,中国(批准号PZSHUTCM200110002）于2020年1月10日。
