Abstract：
Mesenchymal Stem Cells (MSCs) are known to contribute to wound healing by increasing tissue regeneration. This study examined the effect of MSC-Lyophilizate (MSC-L) on the recovery of the zone of stasis in thermal burns.
A comb was used to induce second-degree thermal burns (1 × 2 cm) on the dorsum of the rats. Within 30 min after the burn, MSC-L derived from the umbilical cord was administered to the experimental group and 1.5 ml of 0.9% isotonic sodium chloride to the sham group. The control group did not receive any intervention. Tissue samples were collected on postoperative day 7. Histopathological assessments were made using a microscope with digital camera attachment. SPSS for IBM 25 was used for data analysis.
Epithelial loss and subepidermal bullae were observed in the control and sham groups on day 7. In the experimental group, the MSC-L administration was found to increase epithelial tissue formation and neovascularization in the dermis. We found no significant pathological findings in the epidermis and dermis in the experimental group.
Administration of umbilical cord-derived MSC-L is of potential importance in wound healing. In our study, we observed that MSC-L that contained 1.5 million cells contributed significantly to the recovery of the stasis zone of burn.
A comb was used to induce second-degree thermal burns (1 × 2 cm) on the dorsum of the rats. Within 30 min after the burn, MSC-L derived from the umbilical cord was administered to the experimental group and 1.5 ml of 0.9% isotonic sodium chloride to the sham group. The control group did not receive any intervention. Tissue samples were collected on postoperative day 7. Histopathological assessments were made using a microscope with digital camera attachment. SPSS for IBM 25 was used for data analysis.
Epithelial loss and subepidermal bullae were observed in the control and sham groups on day 7. In the experimental group, the MSC-L administration was found to increase epithelial tissue formation and neovascularization in the dermis. We found no significant pathological findings in the epidermis and dermis in the experimental group.
Administration of umbilical cord-derived MSC-L is of potential importance in wound healing. In our study, we observed that MSC-L that contained 1.5 million cells contributed significantly to the recovery of the stasis zone of burn.
摘要：
已知间充质干细胞(MSC)通过增加组织再生而有助于伤口愈合。这项研究检查了MSC-冻干物（MSC-L）对热烧伤中停滞区恢复的影响。
使用梳子在大鼠的背部诱发二度热烧伤(1×2cm)。烧伤后30分钟内，将源自脐带的MSC-L给予实验组,将1.5ml的0.9%等渗氯化钠给予假手术组。对照组不接受任何干预。在术后第7天收集组织样品。使用带有数码相机附件的显微镜进行组织病理学评估。使用IBM25的SPSS进行数据分析。
在第7天在对照组和假手术组中观察到上皮损失和表皮下大疱。在实验组中,发现MSC-L给药增加真皮中的上皮组织形成和新生血管形成。实验组的表皮和真皮均未发现明显的病理结果。
施用脐带衍生的MSC-L在伤口愈合中具有潜在的重要性。在我们的研究中,我们观察到含有150万个细胞的MSC-L显著有助于烧伤停滞区的恢复。
使用梳子在大鼠的背部诱发二度热烧伤(1×2cm)。烧伤后30分钟内，将源自脐带的MSC-L给予实验组,将1.5ml的0.9%等渗氯化钠给予假手术组。对照组不接受任何干预。在术后第7天收集组织样品。使用带有数码相机附件的显微镜进行组织病理学评估。使用IBM25的SPSS进行数据分析。
在第7天在对照组和假手术组中观察到上皮损失和表皮下大疱。在实验组中,发现MSC-L给药增加真皮中的上皮组织形成和新生血管形成。实验组的表皮和真皮均未发现明显的病理结果。
施用脐带衍生的MSC-L在伤口愈合中具有潜在的重要性。在我们的研究中,我们观察到含有150万个细胞的MSC-L显著有助于烧伤停滞区的恢复。
