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Abstract：
Transcriptomes and DNA methylation of colon cancer at the single-cell level are used to identify marker genes and improve diagnoses and therapies. Seven colon cancer subtypes are recognized based on the single-cell RNA sequence, and the differentially expressed genes regulated by dysregulated methylation are identified as marker genes for different types of colon cancer. Compared with normal colon cells, marker genes of different types show very obvious specificity, especially upregulated genes in tumors. Functional enrichment analysis for marker genes indicates a possible relation between colon cancer and nervous system disease, moreover, the weak immune system is verified in colon cancer. The heightened expression of markers and the reduction of methylation in colon cancer promote tumor development in an extensive mechanism so that there is no biological process that can be enriched in different types.
摘要：
在单细胞水平上，结肠癌的转录组和DNA甲基化用于鉴定标记基因并改善诊断和治疗。根据单细胞RNA序列识别七种结肠癌亚型，甲基化失调调控的差异表达基因被鉴定为不同类型结肠癌的标记基因。与正常结肠细胞相比，不同类型的标记基因表现出非常明显的特异性,尤其是肿瘤中的基因上调。标记基因的功能富集分析表明结肠癌与神经系统疾病之间可能存在关系。此外,弱免疫系统在结肠癌中得到证实。结肠癌中标记物的高度表达和甲基化的减少以广泛的机制促进肿瘤的发展，因此没有可以在不同类型中富集的生物学过程。
