Abstract：
UNASSIGNED: To determine whether electroacupuncture (EA) or moxibustion-like stimulation (MLS) can affect the cutaneous and/or systemic hypothalamic-pituitary-adrenal (HPA) axes.
UNASSIGNED: Rats were divided into Control, EA, 37°C MLS and 43.5°C MLS groups. EA and MLS were performed at bilateral ST36 or LI4. The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. In addition, levels of CRF, ACTH and corticosterone (CORT) in cutaneous tissue and plasma were determined.
UNASSIGNED: Cutaneous expression of CRF, ACTH and GR significantly increased after EA at ST36, while only GR increased after 43.5°C MLS at ST36. The results of EA and MLS at LI4 were in parallel with those at ST36. In plasma, compared with the control group, the level of CORT increased after EA at ST36, while both ACTH and CORT were markedly increased after 43.5°C MLS. For LI4, plasma CRF and CORT increased after EA, while the levels of all three hormones increased following 43.5°C MLS. Notably, compared with the effect of EA, 43.5°C MLS at ST36 produced a more substantial increase in plasma CORT, and 43.5°C MLS at LI4 induced a more dramatic increase in plasma CRF and CORT.
UNASSIGNED: Both EA and 43.5°C MLS can activate the cutaneous and systemic HPA axes of the rat. EA tended to activate the local cutaneous HPA, while 43.5°C MLS was more likely to activate the systemic HPA axis.
UNASSIGNED: Rats were divided into Control, EA, 37°C MLS and 43.5°C MLS groups. EA and MLS were performed at bilateral ST36 or LI4. The expression of corticotropin-releasing factor (CRF), adrenocorticotropic hormone (ACTH) and the glucocorticoid receptor (GR) was detected in local cutaneous tissues at the site of ST36 and LI4 by immunohistochemical staining. In addition, levels of CRF, ACTH and corticosterone (CORT) in cutaneous tissue and plasma were determined.
UNASSIGNED: Cutaneous expression of CRF, ACTH and GR significantly increased after EA at ST36, while only GR increased after 43.5°C MLS at ST36. The results of EA and MLS at LI4 were in parallel with those at ST36. In plasma, compared with the control group, the level of CORT increased after EA at ST36, while both ACTH and CORT were markedly increased after 43.5°C MLS. For LI4, plasma CRF and CORT increased after EA, while the levels of all three hormones increased following 43.5°C MLS. Notably, compared with the effect of EA, 43.5°C MLS at ST36 produced a more substantial increase in plasma CORT, and 43.5°C MLS at LI4 induced a more dramatic increase in plasma CRF and CORT.
UNASSIGNED: Both EA and 43.5°C MLS can activate the cutaneous and systemic HPA axes of the rat. EA tended to activate the local cutaneous HPA, while 43.5°C MLS was more likely to activate the systemic HPA axis.
摘要：
■确定电针（EA）或艾灸样刺激（MLS）是否会影响皮肤和/或全身下丘脑-垂体-肾上腺（HPA）轴。
■将大鼠分为对照组，EA,37°CMLS和43.5°CMLS组。EA和MLS在双侧ST36或LI4进行。促肾上腺皮质激素释放因子(CRF)的表达,通过免疫组织化学染色在ST36和LI4部位的局部皮肤组织中检测到促肾上腺皮质激素（ACTH）和糖皮质激素受体（GR）。此外,CRF的水平，测定皮肤组织和血浆中的ACTH和皮质酮（CORT）。
■CRF的皮肤表达，在ST36的EA后，ACTH和GR显着增加，而在ST36的43.5°CMLS后，只有GR增加。在LI4的EA和MLS的结果与在ST36的结果平行。在等离子体中，与对照组相比，在ST36时EA后CORT水平升高，而在43.5°CMLS后ACTH和CORT均显着升高。对于LI4，EA后血浆CRF和CORT增加，而所有三种激素的水平在43.5°CMLS后增加。值得注意的是,与EA的效果相比，ST36的43.5°CMLS产生了更大幅度的血浆CORT增加，LI4和43.5°CMLS诱导血浆CRF和CORT更显著增加。
■EA和43.5°CMLS均可激活大鼠的皮肤和全身HPA轴。EA倾向于激活局部皮肤HPA，而43.5°CMLS更有可能激活全身HPA轴。
■将大鼠分为对照组，EA,37°CMLS和43.5°CMLS组。EA和MLS在双侧ST36或LI4进行。促肾上腺皮质激素释放因子(CRF)的表达,通过免疫组织化学染色在ST36和LI4部位的局部皮肤组织中检测到促肾上腺皮质激素（ACTH）和糖皮质激素受体（GR）。此外,CRF的水平，测定皮肤组织和血浆中的ACTH和皮质酮（CORT）。
■CRF的皮肤表达，在ST36的EA后，ACTH和GR显着增加，而在ST36的43.5°CMLS后，只有GR增加。在LI4的EA和MLS的结果与在ST36的结果平行。在等离子体中，与对照组相比，在ST36时EA后CORT水平升高，而在43.5°CMLS后ACTH和CORT均显着升高。对于LI4，EA后血浆CRF和CORT增加，而所有三种激素的水平在43.5°CMLS后增加。值得注意的是,与EA的效果相比，ST36的43.5°CMLS产生了更大幅度的血浆CORT增加，LI4和43.5°CMLS诱导血浆CRF和CORT更显著增加。
■EA和43.5°CMLS均可激活大鼠的皮肤和全身HPA轴。EA倾向于激活局部皮肤HPA，而43.5°CMLS更有可能激活全身HPA轴。
