Abstract：
Good Cell and Tissue Culture Practice (GCCP) 2.0 is an updated guidance document from GCCP 1.0 (published by ECVAM in 2005), which was developed for practical use in the laboratory to assure the reproducibility of in vitro (cell-based) work. The update in the guidance was essential as cell models have advanced dramatically to more complex culture systems and need more comprehensive quality management to ensure reproducibility and high-quality scientific data. This document describes six main principles to consider when performing cell culture including characterization and maintenance of essential characteristics, quality management, documentation and reporting, safety, education and training, and ethics. The document does not intend to impose detailed procedures but to describe potential quality issues. It is foreseen that the document will require further updates as the science and technologies evolve over time.
摘要：
良好的细胞和组织培养规范（GCCP）2.0是GCCP1.0（由ECVAM于2005年发布）的最新指导文件，这是为了在实验室中实际使用而开发的，以确保体外（基于细胞）工作的可重复性。指南的更新是必不可少的，因为细胞模型已经大大推进到更复杂的培养系统，需要更全面的质量管理，以确保再现性和质量衍生的科学数据。本文描述了进行细胞培养时要考虑的六个主要原则，包括表征和维持基本特征，质量管理,文档和报告，安全,教育和培训,和道德。该文件不打算强加详细的程序，而是描述潜在的质量问题。可以预见，随着科学技术的发展，该文件将需要进一步更新。
