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Abstract：
Periodontitis is a globally prevalent disease that imposes a functional and aesthetic burden on patients. The oral microbiome influences human health. The aim of this study was at assessing gender variation in the subgingival bacterial microbiome of elderly patients with initial periodontitis and to determine the causes of this variation. Twelve males and twenty females (range 50-68 years old) with initial periodontitis provided subgingival plaque samples. 16S rRNA gene sequencing, QIIME-based data processing, and statistical analyses were carried out using several different analytical approaches to detect differences in the oral microbiome between the two groups. Males had higher Chao1 index, observed species, and phylogenetic diversity whole tree values than females. Analysis of β-diversity indicated that the samples were reasonably divided by the gender. The linear discriminant analysis effect size showed that the most representative biomarkers were the genus Haemophilus in males, whereas the dominant bacteria in females were Campylobacter. Kyoto Encyclopedia of Genes and Genomes analysis showed that predicting changes in the female oral microbiota may be related to the immune system and immune system diseases are the main factor in males. These data suggest that gender may be a differentiating factor in the microbial composition of subgingival plaques in elderly patients with initial periodontitis. These results could deepen our understanding of the role of gender in the oral microbiota present during initial periodontitis.
摘要：
牙周炎是一种全球流行的疾病，给患者带来功能和美学负担。口腔微生物组影响人类健康。这项研究的目的是评估患有初始牙周炎的老年患者的龈下细菌微生物组的性别变化，并确定这种变化的原因。患有初始牙周炎的十二名男性和二十名女性（年龄在50-68岁之间）提供了龈下菌斑样本。16SrRNA基因测序，基于QIIME的数据处理，使用几种不同的分析方法进行统计分析，以检测两组之间口腔微生物组的差异。男性的Chao1指数较高，观察到的物种，和系统发育多样性全树价值比雌性。β多样性分析表明，样本按性别划分合理。线性判别分析效应大小显示,最具代表性的生物标志物是雄性嗜血杆菌属,而女性的优势细菌是弯曲杆菌。《京都百科全书》的基因和基因组分析表明，预测女性口腔微生物群的变化可能与免疫系统有关，免疫系统疾病是男性的主要因素。这些数据表明，性别可能是患有初始牙周炎的老年患者龈下菌斑微生物组成的区别因素。这些结果可以加深我们对性别在初始牙周炎期间口腔微生物群中的作用的理解。
