PDF(Pubmed)
Abstract：
UNASSIGNED: Sebocytes are the main cells involved in the pathogenesis of acne by producing lipids and inflammatory cytokines. Although palmitic acid (PA) has been suggested to induce an inflammatory reaction, its effect on sebocytes remains to be elucidated.
UNASSIGNED: In the present study, we investigated whether PA promotes inflammasome-mediated inflammation of sebocytes both in vivo and in vitro.
UNASSIGNED: We intradermally injected PA into the mice ears. And, we treated cultured human sebocytes with PA. Inflammasome-mediated inflammation was verified by immunohistochemistry, Western blot and ELISA.
UNASSIGNED: PA-treated mice developed an inflammatory response associated with increased interleukin (IL)-1β expression in the sebaceous glands. When PA was added to cultured human sebocytes, caspase-1 activation and IL-1β secretion were significantly enhanced. In addition, NLRP3 knockdown attenuated IL-1β production by sebocytes stimulated with PA. PA-mediated inflammasome activation required reactive oxygen species.
UNASSIGNED: These findings indicate that PA activates the NLRP3 inflammasome before induction of an inflammatory response in sebocytes. Thus, PA may play a role in the inflammation of acne.
UNASSIGNED: In the present study, we investigated whether PA promotes inflammasome-mediated inflammation of sebocytes both in vivo and in vitro.
UNASSIGNED: We intradermally injected PA into the mice ears. And, we treated cultured human sebocytes with PA. Inflammasome-mediated inflammation was verified by immunohistochemistry, Western blot and ELISA.
UNASSIGNED: PA-treated mice developed an inflammatory response associated with increased interleukin (IL)-1β expression in the sebaceous glands. When PA was added to cultured human sebocytes, caspase-1 activation and IL-1β secretion were significantly enhanced. In addition, NLRP3 knockdown attenuated IL-1β production by sebocytes stimulated with PA. PA-mediated inflammasome activation required reactive oxygen species.
UNASSIGNED: These findings indicate that PA activates the NLRP3 inflammasome before induction of an inflammatory response in sebocytes. Thus, PA may play a role in the inflammation of acne.
摘要：
■皮脂腺细胞是通过产生脂质和炎性细胞因子而参与痤疮发病的主要细胞。虽然棕榈酸（PA）已被建议诱导炎症反应，它对皮脂腺细胞的影响还有待阐明。
■在本研究中，我们研究了PA是否在体内和体外促进炎症小体介导的皮脂腺细胞炎症。
■我们将PA皮内注射到小鼠耳朵中。And,我们用PA处理培养的人皮脂细胞。炎症小体介导的炎症通过免疫组织化学证实，Westernblot和ELISA。
■PA处理的小鼠出现了与皮脂腺中白细胞介素（IL）-1β表达增加相关的炎症反应。当PA添加到培养的人皮脂腺细胞中时，caspase-1激活和IL-1β分泌显著增强。此外,NLRP3敲低减弱了用PA刺激的皮脂细胞产生的IL-1β。PA介导的炎性小体活化需要活性氧。
■这些发现表明，PA在诱导皮脂腺细胞的炎症反应之前激活了NLRP3炎性体。因此，PA可能在痤疮的炎症中起作用。
■在本研究中，我们研究了PA是否在体内和体外促进炎症小体介导的皮脂腺细胞炎症。
■我们将PA皮内注射到小鼠耳朵中。And,我们用PA处理培养的人皮脂细胞。炎症小体介导的炎症通过免疫组织化学证实，Westernblot和ELISA。
■PA处理的小鼠出现了与皮脂腺中白细胞介素（IL）-1β表达增加相关的炎症反应。当PA添加到培养的人皮脂腺细胞中时，caspase-1激活和IL-1β分泌显著增强。此外,NLRP3敲低减弱了用PA刺激的皮脂细胞产生的IL-1β。PA介导的炎性小体活化需要活性氧。
■这些发现表明，PA在诱导皮脂腺细胞的炎症反应之前激活了NLRP3炎性体。因此，PA可能在痤疮的炎症中起作用。
