PDF(Pubmed)
Abstract：
Human papillomaviruses (HPV) are non-enveloped DNA viruses infecting cutaneous and mucosal squamous epithelia. Sexually transmitted HPV-types that are carcinogenic to humans such as HPV16 can induce cervical and other anogenital cancers. Virus transmission through fomites such as inadequately disinfected gynecological equipment is a further potential transmission route. Since HPV cannot be easily grown in cell culture, polyomavirus SV40 has been used as a surrogate virus when testing the virucidal activity of chemical disinfectants. So far, studies that have compared the virucidal activity of different disinfectants against HPV and SV40 are lacking. Here, we evaluated the susceptibility of HPV16 pseudovirus and SV40 to seven active biocidal substances using quantitative suspension tests. Ethanol, glutaraldehyde (GTA), dodecyldipropylentriamin (DPTA), and ortho-phthalaldehydes (OPA) were able to reduce the infectivity of HPV16 pseudovirus >99.99% after 5 min. In contrast, isopropanol, peracetic acid (PAA), and quaternary ammonium compounds with alkylamines (QAC) only led to a slight or no reduction in infectivity. Concerning SV40, only GTA (60 min contact time), PAA, and OPA had virus-inactivating effects. In conclusion, the virucidal activity of three out of seven disinfectants tested was different for HPV16 pseudovirus and SV40. In this study, SV40 was shown to be a reliable surrogate virus for HPV when testing isopropanol-, GTA-, QAC-, and OPA-based disinfectants.
摘要：
人乳头瘤病毒（HPV）是感染皮肤和粘膜鳞状上皮的无包膜DNA病毒。对人类致癌的性传播HPV类型如HPV16可诱导子宫颈癌和其他肛门生殖器癌。通过诸如消毒不充分的妇科设备之类的食物进行病毒传播是进一步的潜在传播途径。由于HPV不容易在细胞培养物中生长，在测试化学消毒剂的杀病毒活性时，多瘤病毒SV40已被用作替代病毒。到目前为止,缺乏比较不同消毒剂对HPV和SV40的杀病毒活性的研究。这里,我们使用定量悬浮试验评估了HPV16假病毒和SV40对7种活性杀生物物质的易感性.乙醇,戊二醛（GTA），十二烷基二丙基三胺（DPTA），5分钟后，邻苯二醛（OPA）能够降低HPV16假病毒的感染性>99.99％。相比之下,异丙醇,过乙酸(PAA),和季铵化合物与烷基胺（QAC）仅导致感染性略有降低或没有降低。关于SV40，仅GTA（60分钟接触时间），PAA,OPA有病毒灭活作用。总之,所测试的7种消毒剂中的3种对HPV16假病毒和SV40的杀病毒活性不同。在这项研究中,在检测异丙醇时，SV40被证明是HPV的可靠替代病毒，GTA-,QAC-,和基于OPA的消毒剂。
