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Abstract：
UNASSIGNED: Failure of fascial healing in the abdominal wall can result in incisional hernia, which is one of the most common complications after laparotomy. Understanding the molecular healing process of abdominal fascia may provide lipid markers of incisional hernia or therapeutic targets that allow prevention or treatment of incisional hernias.
UNASSIGNED: This study aims to investigate temporal and in situ changes of lipids during the normal healing process of abdominal fascia in the first postoperative week.
UNASSIGNED: Open hemicolectomy was performed in a total of 35 Wistar rats. The midline fascia was closed identically for all rats using a single continuous suturing technique. These animals were sacrificed with equal numbers (n = 5) at each of 7-time points (6, 12, 24, 48, 72, 120, and 168 h. The local and temporal changes of lipids were examined with mass spectrometry imaging and correlated to histologically scored changes during healing using hematoxylin and eosin staining.
UNASSIGNED: Two phosphatidylcholine lipid species (PC O-38:5 and PC 38:4) and one phosphatidylethanolamine lipid (PE O-16:1_20:4) were found to significantly correlate with temporal changes of inflammation. A phosphatidylcholine (PC 32:0) and a monosialodihexosylganglioside (GM3 34:1;2) were found to correlate with fibroblast cell growth.
UNASSIGNED: Glycerophospholipids and gangliosides are strongly involved in the normal healing process of abdominal fascia and their locally fluctuating concentrations are considered as potential lipid markers and therapeutic targets of fascial healing.
UNASSIGNED: This study aims to investigate temporal and in situ changes of lipids during the normal healing process of abdominal fascia in the first postoperative week.
UNASSIGNED: Open hemicolectomy was performed in a total of 35 Wistar rats. The midline fascia was closed identically for all rats using a single continuous suturing technique. These animals were sacrificed with equal numbers (n = 5) at each of 7-time points (6, 12, 24, 48, 72, 120, and 168 h. The local and temporal changes of lipids were examined with mass spectrometry imaging and correlated to histologically scored changes during healing using hematoxylin and eosin staining.
UNASSIGNED: Two phosphatidylcholine lipid species (PC O-38:5 and PC 38:4) and one phosphatidylethanolamine lipid (PE O-16:1_20:4) were found to significantly correlate with temporal changes of inflammation. A phosphatidylcholine (PC 32:0) and a monosialodihexosylganglioside (GM3 34:1;2) were found to correlate with fibroblast cell growth.
UNASSIGNED: Glycerophospholipids and gangliosides are strongly involved in the normal healing process of abdominal fascia and their locally fluctuating concentrations are considered as potential lipid markers and therapeutic targets of fascial healing.
摘要：
■腹壁筋膜愈合失败可导致切口疝，这是开腹手术后最常见的并发症之一。了解腹部筋膜的分子愈合过程可能会提供切口疝的脂质标志物或治疗靶标，从而可以预防或治疗切口疝。
■本研究旨在研究术后第一周腹部筋膜正常愈合过程中脂质的时间和原位变化。
■在总共35只Wistar大鼠中进行开放半结肠切除术。使用单个连续缝合技术将所有大鼠的中线筋膜相同地闭合。在7个时间点(6、12、24、48、72、120和168小时)中的每一个以相等数量(n=5)处死这些动物。用质谱成像检查脂质的局部和时间变化,并使用苏木精和曙红染色与愈合期间的组织学评分变化相关联。
■发现两种磷脂酰胆碱脂质（PCO-38：5和PC38：4）和一种磷脂酰乙醇胺脂质（PEO-16：1_20：4）与炎症的时间变化显着相关。发现磷脂酰胆碱（PC32：0）和单唾液酸二己糖神经节苷脂（GM334：1;2）与成纤维细胞生长相关。
■甘油磷脂和神经节苷脂强烈参与腹部筋膜的正常愈合过程，其局部波动浓度被认为是筋膜愈合的潜在脂质标志物和治疗目标。
■本研究旨在研究术后第一周腹部筋膜正常愈合过程中脂质的时间和原位变化。
■在总共35只Wistar大鼠中进行开放半结肠切除术。使用单个连续缝合技术将所有大鼠的中线筋膜相同地闭合。在7个时间点(6、12、24、48、72、120和168小时)中的每一个以相等数量(n=5)处死这些动物。用质谱成像检查脂质的局部和时间变化,并使用苏木精和曙红染色与愈合期间的组织学评分变化相关联。
■发现两种磷脂酰胆碱脂质（PCO-38：5和PC38：4）和一种磷脂酰乙醇胺脂质（PEO-16：1_20：4）与炎症的时间变化显着相关。发现磷脂酰胆碱（PC32：0）和单唾液酸二己糖神经节苷脂（GM334：1;2）与成纤维细胞生长相关。
■甘油磷脂和神经节苷脂强烈参与腹部筋膜的正常愈合过程，其局部波动浓度被认为是筋膜愈合的潜在脂质标志物和治疗目标。
