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Abstract：
BACKGROUND: Korean Red Ginseng (KRG) is a traditional herb that has several beneficial properties including anti-aging, anti-inflammatory, and autophagy regulatory effects. However, the mechanisms of these effects are not well understood. In this report, the underlying mechanisms of anti-inflammatory and autophagy-promoting effects were investigated in aged mice treated with KRG-water extract (WE) over a long period.
METHODS: The mechanisms of anti-inflammatory and autophagy-promoting activities of KRG-WE were evaluated in kidney, lung, liver, stomach, and colon of aged mice using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), and western blot analysis.
RESULTS: KRG-WE significantly suppressed the mRNA expression levels of inflammation-related genes such as interleukin (IL)-1β, IL-8, tumor necrosis factor (TNF)-α, monocyte chemoattractant protein-1 (MCP-1), and IL-6 in kidney, lung, liver, stomach, and colon of the aged mice. Furthermore, KRG-WE downregulated the expression of transcription factors and their protein levels associated with inflammation in lung and kidney of aged mice. KRG-WE also increased the expression of autophagy-related genes and their protein levels in colon, liver, and stomach.
CONCLUSIONS: The results suggest that KRG can suppress inflammatory responses and recover autophagy activity in aged mice.
METHODS: The mechanisms of anti-inflammatory and autophagy-promoting activities of KRG-WE were evaluated in kidney, lung, liver, stomach, and colon of aged mice using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), and western blot analysis.
RESULTS: KRG-WE significantly suppressed the mRNA expression levels of inflammation-related genes such as interleukin (IL)-1β, IL-8, tumor necrosis factor (TNF)-α, monocyte chemoattractant protein-1 (MCP-1), and IL-6 in kidney, lung, liver, stomach, and colon of the aged mice. Furthermore, KRG-WE downregulated the expression of transcription factors and their protein levels associated with inflammation in lung and kidney of aged mice. KRG-WE also increased the expression of autophagy-related genes and their protein levels in colon, liver, and stomach.
CONCLUSIONS: The results suggest that KRG can suppress inflammatory responses and recover autophagy activity in aged mice.
摘要：
背景：韩国红参（KRG）是一种传统草药，具有多种有益特性，包括抗衰老，抗炎,和自噬调节作用。然而,这些效应的机制还没有得到很好的理解。在这份报告中,在长期接受KRG-水提取物(WE)治疗的老年小鼠中研究了抗炎和促进自噬作用的潜在机制.
方法：在肾脏中评估KRG-WE的抗炎和促进自噬活性的机制，肺，肝脏,胃,使用半定量逆转录聚合酶链反应（RT-PCR），定量RT-PCR（qRT-PCR），和蛋白质印迹分析。
结果：KRG-WE显著抑制炎症相关基因如白细胞介素（IL）-1β的mRNA表达，IL-8,肿瘤坏死因子(TNF)-α,单核细胞趋化蛋白-1（MCP-1），和肾脏中的IL-6，肺，肝脏,胃,和老年小鼠的结肠。此外，KRG-WE下调老年小鼠肺和肾脏炎症相关转录因子及其蛋白水平的表达.KRG-WE还增加了结肠中自噬相关基因的表达及其蛋白水平，肝脏,和胃。
结论：结果表明，KRG可以抑制老年小鼠的炎症反应并恢复自噬活性。
方法：在肾脏中评估KRG-WE的抗炎和促进自噬活性的机制，肺，肝脏,胃,使用半定量逆转录聚合酶链反应（RT-PCR），定量RT-PCR（qRT-PCR），和蛋白质印迹分析。
结果：KRG-WE显著抑制炎症相关基因如白细胞介素（IL）-1β的mRNA表达，IL-8,肿瘤坏死因子(TNF)-α,单核细胞趋化蛋白-1（MCP-1），和肾脏中的IL-6，肺，肝脏,胃,和老年小鼠的结肠。此外，KRG-WE下调老年小鼠肺和肾脏炎症相关转录因子及其蛋白水平的表达.KRG-WE还增加了结肠中自噬相关基因的表达及其蛋白水平，肝脏,和胃。
结论：结果表明，KRG可以抑制老年小鼠的炎症反应并恢复自噬活性。
