Abstract：
BACKGROUND: Signal recognition particle (SRP) promotes co-translational translocation of the proteins through or into the endoplasmic reticulum membrane and it also has elongation arrest function. SRP9 is one of the six protein subunits of SRP and functions in elongation arrest activity by forming a heterodimeric structure with SRP14. It is one of the substrates of ADAR, which has been found to have a role in breast cancer. This study was conducted to investigate the SRP9 protein expression in normal and tumor tissues of patients with breast cancer and determine its prognostic significance.
RESULTS: A total of 32 female patients who were diagnosed as having primary breast cancer and underwent surgery were included in the study. Western Blotting was performed to detect SRP9 protein expression levels in normal and tumor tissue samples. Clinical and pathologic characteristics were analyzed to assess the prognostic significance. SRP9 protein expression was statistically higher in the breast cancer tissue samples compared to normal matched tissue, and the mean SRP9 protein expression levels of breast cancer tissue normal tissue samples were 1.019 ± 1.011 and 0.551 ± 0.456, respectively (p = 0.001). SRP9 protein expression levels in tumor tissue of patients with lymph node metastasis, tumor size > 2 cm, estrogen receptor-positive, progesterone receptor-positive, and HER-2 negative were statistically higher than in normal tissue (p < 0.05).
CONCLUSIONS: It is vital to clarify the roles of molecules such as SRP9 in understanding the pathogenesis of breast cancer. In our study, we showed that SRP9 expression increased in breast cancer and was associated with disease-related parameters.
RESULTS: A total of 32 female patients who were diagnosed as having primary breast cancer and underwent surgery were included in the study. Western Blotting was performed to detect SRP9 protein expression levels in normal and tumor tissue samples. Clinical and pathologic characteristics were analyzed to assess the prognostic significance. SRP9 protein expression was statistically higher in the breast cancer tissue samples compared to normal matched tissue, and the mean SRP9 protein expression levels of breast cancer tissue normal tissue samples were 1.019 ± 1.011 and 0.551 ± 0.456, respectively (p = 0.001). SRP9 protein expression levels in tumor tissue of patients with lymph node metastasis, tumor size > 2 cm, estrogen receptor-positive, progesterone receptor-positive, and HER-2 negative were statistically higher than in normal tissue (p < 0.05).
CONCLUSIONS: It is vital to clarify the roles of molecules such as SRP9 in understanding the pathogenesis of breast cancer. In our study, we showed that SRP9 expression increased in breast cancer and was associated with disease-related parameters.
摘要：
背景：信号识别颗粒（SRP）促进蛋白质通过或进入内质网膜的共翻译易位，并且还具有延伸停滞功能。SRP9是SRP的六个蛋白质亚基之一，通过与SRP14形成异二聚体结构而在伸长停滞活性中起作用。它是ADAR的底物之一，已被发现在乳腺癌中起作用。本研究旨在探讨SRP9蛋白在乳腺癌患者正常组织和肿瘤组织中的表达及其预后意义。
结果：共有32名诊断为原发性乳腺癌并接受手术的女性患者纳入研究。进行蛋白质印迹以检测正常和肿瘤组织样品中的SRP9蛋白表达水平。分析临床和病理特征以评估预后意义。乳腺癌组织样本中SRP9蛋白表达高于正常组织，乳腺癌组织正常组织样本的平均SRP9蛋白表达水平分别为1.019±1.011和0.551±0.456(p=0.001)。淋巴结转移患者肿瘤组织中SRP9蛋白表达水平,肿瘤大小>2厘米，雌激素受体阳性，孕激素受体阳性,而HER-2阴性的组织均高于正常组织(p<0.05)。
结论：阐明SRP9等分子在理解乳腺癌发病机制中的作用至关重要。在我们的研究中,我们发现SRP9在乳腺癌中的表达增加,并且与疾病相关参数相关.
结果：共有32名诊断为原发性乳腺癌并接受手术的女性患者纳入研究。进行蛋白质印迹以检测正常和肿瘤组织样品中的SRP9蛋白表达水平。分析临床和病理特征以评估预后意义。乳腺癌组织样本中SRP9蛋白表达高于正常组织，乳腺癌组织正常组织样本的平均SRP9蛋白表达水平分别为1.019±1.011和0.551±0.456(p=0.001)。淋巴结转移患者肿瘤组织中SRP9蛋白表达水平,肿瘤大小>2厘米，雌激素受体阳性，孕激素受体阳性,而HER-2阴性的组织均高于正常组织(p<0.05)。
结论：阐明SRP9等分子在理解乳腺癌发病机制中的作用至关重要。在我们的研究中,我们发现SRP9在乳腺癌中的表达增加,并且与疾病相关参数相关.
