Abstract：
Flow cytometry is a powerful technology used in research, drug development and clinical sample analysis for cell identification and characterization, allowing for the simultaneous interrogation of multiple targets on various cell subsets from limited samples. Recent advancements in instrumentation and fluorochrome availability have resulted in significant increases in the complexity and dimensionality of flow cytometry panels. Though this increase in panel size allows for detection of a broader range of markers and sub-populations, even in restricted biological samples, it also comes with many challenges in panel design, optimization, and downstream data analysis and interpretation. In the current paper we describe the practices we established for development of high-dimensional panels on the Aurora spectral flow cytometer to aid clinical sample analysis.
摘要：
流式细胞术是一项用于研究的强大技术，用于细胞鉴定和表征的药物开发和临床样品分析，允许同时询问来自有限样品的各种细胞亚群上的多个靶标。仪器和荧光染料可用性的最新进展导致流式细胞仪面板的复杂性和维度显着增加。尽管面板大小的这种增加允许检测更广泛的标记和亚群，即使在有限的生物样本中，它在面板设计方面也带来了许多挑战，优化，和下游数据分析和解释。在本文中，我们描述了在Aurora光谱流式细胞仪上开发高维面板以帮助临床样品分析的实践。
