PDF(Pubmed)
Abstract：
The development of periodontitis is associated with an imbalanced subgingival microbial community enriched with species such as the traditionally classified red-complex bacteria (Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola). Saliva has been suggested as an alternative to subgingival plaque for the microbial analysis due to its easy and non-invasive collection. This systematic review aims to determine whether the levels of red-complex bacteria assessed using saliva reflect those in subgingival plaque from periodontitis patients. The MEDLINE, EMBASE, and Cochrane Library databases were searched up to April 30, 2021. Studies were considered eligible if microbial data of at least one of the red-complex species were reported in both saliva and subgingival plaque from periodontitis patients, based on DNA-based methods. Of the 17 included studies, 4 studies used 16S rRNA gene sequencing techniques, and the rest used PCR-based approaches. The detection frequency of each red-complex species in periodontitis patients was reported to be > 60% in most studies, irrespective of samples types. Meta-analyses revealed that both detection frequencies and relative abundances of red-complex bacteria in saliva were significantly lower than those in subgingival plaque. Moreover, the relative abundances of all 3 bacterial species in saliva showed significantly positive correlation with those in subgingival plaque. In conclusion, current evidence suggests that one-time saliva sampling cannot replace subgingival plaque for microbial analysis of the red-complex bacteria in periodontitis patients. Given the positive microbial associations between saliva and subgingival plaque, a thorough review of longitudinal clinical studies is needed to further assess the role of saliva.
摘要：
牙周炎的发展与不平衡的龈下微生物群落有关，该群落富含诸如传统分类的红色复合细菌（牙龈卟啉单胞菌，连翘坦菌,和Denticola密螺旋体）。唾液由于其易于和非侵入性收集，已被建议作为牙龈下菌斑的替代品进行微生物分析。本系统综述旨在确定使用唾液评估的红色复合细菌水平是否反映了牙周炎患者牙龈下菌斑中的红色复合细菌水平。MEDLINE,EMBASE,到2021年4月30日，搜索了Cochrane图书馆数据库。如果在牙周炎患者的唾液和龈下菌斑中报告了至少一种红色复合物物种的微生物数据，则认为研究合格。基于DNA的方法。在17项纳入的研究中，4项研究使用16SrRNA基因测序技术，其余使用基于PCR的方法。据报道，在大多数研究中，牙周炎患者中每种红色复合物的检测频率均>60％，无论样品类型。荟萃分析表明，唾液中红色复合细菌的检测频率和相对丰度均显着低于龈下菌斑。此外，唾液中所有3种细菌的相对丰度与龈下菌斑中的细菌呈显著正相关。总之,目前的证据表明,一次性唾液取样不能替代牙龈下菌斑,用于牙周炎患者红色复合细菌的微生物分析.鉴于唾液和龈下菌斑之间的微生物正相关，需要对纵向临床研究进行全面审查,以进一步评估唾液的作用.
