PDF(Pubmed)
Abstract：
Microcirculation in vivo has been assessed using non-invasive technologies such as laser Doppler flowmetry (LDF). In contrast to chronic hyperglycemia, known to induce microvascular dysfunction, the effects of short-term elevations in blood glucose on microcirculation are controversial. We aimed to assess the impact of an oral glucose load (OGL) on the cutaneous microcirculation of healthy subjects, quantified by LDF and coupled with wavelet transform (WT) as an interpretation tool. On two separate occasions, sixteen subjects drank either a glucose solution (75 g in 250 mL water) or water (equal volume). LDF signals were obtained in two anatomical sites (forearm and finger pulp) before and after each load (pre-load and post-load, respectively), in resting conditions and during post-occlusive reactive hyperemia (PORH). The WT allowed decomposition of the LDF signals into their spectral components (cardiac, respiratory, myogenic, sympathetic, endothelial NO-dependent). The OGL blunted the PORH response in the forearm, which was not observed with the water load. Significant differences were found for the cardiac and sympathetic components in the glucose and water groups between the pre-load and post-load periods. These results suggest that an OGL induces a short-term subtle microvascular impairment, probably involving a modulation of the sympathetic nervous system.
摘要：
已使用非侵入性技术例如激光多普勒血流仪(LDF)评估了体内微循环。与慢性高血糖相反，已知会诱发微血管功能障碍，短期血糖升高对微循环的影响存在争议.我们旨在评估口服葡萄糖负荷（OGL）对健康受试者皮肤微循环的影响，通过LDF量化，并结合小波变换(WT)作为解释工具。在两个不同的场合，16名受试者饮用葡萄糖溶液(75g在250mL水中)或水(等体积).在每次加载之前和之后（加载前和加载后，在两个解剖部位（前臂和指腹）获得LDF信号，分别)，在静息状态和闭塞后反应性充血（PORH）期间。WT允许将LDF信号分解为其频谱分量(心脏，呼吸,生肌,同情,内皮NO依赖性）。OGL减弱了前臂的PORH反应，这在水负荷下没有观察到。在负荷前和负荷后阶段之间，葡萄糖和水组的心脏和交感神经成分存在显着差异。这些结果表明，OGL诱导短期细微的微血管损伤，可能涉及交感神经系统的调制。
