PDF(Pubmed)
Abstract：
Fatty acid (FA) composition is one of the most important parameters for the assessment of meat quality in pigs. The FA composition in pork can also affect human health. Our aim was to identify quantitative trait loci (QTLs) and positional candidate genes affecting the FA profile of the longissimus dorsi muscle in a large F2 intercross between Landrace and Korean native pigs comprising 1105 F2 progeny by genome-wide association studies (GWAS) and post-GWAS high-resolution mapping analyses. We performed GWAS using the PorcineSNP60K BeadChip and a linear mixed model. Four genome-wide significant QTL regions in SSC8, SSC12, SSC14, and SSC16 were detected (p < 2.53 × 10-7). Several co-localizations of QTLs in SSC12 for oleic acid, linoleic acid, arachidonic acid, monounsaturated FAs, polyunsaturated FAs, and the polyunsaturated/saturated FA ratio were observed. To refine the QTL region in SSC12, a linkage and linkage disequilibrium analysis was applied and could narrow down the critical region to a 0.749 Mb region. Of the genes in this region, GAS7, MYH2, and MYH3 were identified as strong novel candidate genes based on further conditional association analyses. These findings provide a novel insight into the genetic basis of FA composition in pork and could contribute to the improvement of pork quality.
摘要：
脂肪酸（FA）组成是评估猪肉质的最重要参数之一。猪肉中的FA成分也会影响人体健康。我们的目的是通过全基因组关联研究（GWAS）和GWAS后高分辨率作图分析，在长白猪和韩国本地猪之间的大型F2杂交中，确定影响背肌FA谱的数量性状基因座（QTL）和位置候选基因。我们使用PorcineSNP60KBeadChip和线性混合模型进行GWAS。在SSC8，SSC12，SSC14和SSC16中检测到四个全基因组重要的QTL区域（p<2.53×10-7）。SSC12中QTL对油酸的几种共定位，亚油酸,花生四烯酸,单不饱和脂肪酸，多不饱和脂肪酸，观察到多不饱和/饱和FA比。为了细化SSC12中的QTL区域，应用了连锁和连锁不平衡分析，可以将关键区域缩小到0.749Mb区域。在这个地区的基因中，基于进一步的条件关联分析,GAS7、MYH2和MYH3被鉴定为强的新候选基因。这些发现为猪肉中FA组成的遗传基础提供了新的见解，并可能有助于改善猪肉品质。
