Abstract：
Hedgehog signaling pathway shapes our body by regulating proliferation and differentiation of cells. The spatial and temporal distribution pattern of its ligands finely controls the activity of the Hedgehog pathway during development. To mimic the active regulation of Hedgehog pathway, we have developed a light-inducible Hedgehog signaling activator 6-nitroveratryloxy-carbonyl Smoothened agonist (NVOC-SAG). Here we describe a method to selectively induce ventral differentiation of human iPS cell-derived forebrain organoids in a light-dependent manner. This article describes preparation of NVOC-SAG, culture of iPS cell-derived forebrain organoids, light irradiation, and downstream analyses.
摘要：
Hedgehog信号通路通过调节细胞的增殖和分化来塑造我们的身体。其配体的时空分布模式精细地控制了发育过程中Hedgehog途径的活性。模拟Hedgehog通路的主动调节,我们已经开发了一种光诱导的Hedgehog信号激活剂6-硝基veratryloxy-羰基平滑激动剂（NVOC-SAG）。在这里，我们描述了一种以光依赖性方式选择性诱导人iPS细胞衍生的前脑类器官的腹侧分化的方法。本文介绍了NVOC-SAG的制备，iPS细胞来源的前脑类器官的培养，光照射，和下游分析。
