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Abstract：
The microfluidic paper-based analytical devices (μPADs) have grown-up swiftly over the decade due to its low cost, simple fabrication procedure, resource-limitedness, non-toxicity and their environmentally benign nature. The μPADs, also identified as point-of-care devices or health care devices have successfully applied in several fields such as diagnostics, biological, food safety, environmental, electrochemical and most importantly colorimetric/fluorimetric sensors, owing to the attractive passive motions of analyte without any external forces. In recent years, a large number of colorimetric and fluorimetric probes have been reported that can selectively recognize the analytes in μPADs. However, there is no organized review on its structure-activity relationship. In this review, we have focused to summarize the colorimetric and fluorimetric probes utilized in μPADs. This review discuss about the relationships between the structure and functions of various probes as signaling units of the efficient μPADs. The probes including nanomaterials, nanozymes, polymers and organic molecules, their structural activity with regard to sensing performances along with their limit of detection are also discussed. This review is expected to assist readers for better understanding of the sensing mechanisms of various chemo and bio-probes utilized in μPADs, as well as promote their advancement in the field. On the other hand, this review also helps the researchers for enhancement of μPADs and paves way for synergistic application of existing molecular probes as an effective diagnostic tool for the worldwide pandemic novel corona virus COVID-19.
摘要：
微流控纸基分析设备（μPAD）由于其低成本在过去十年中迅速成长起来，简单的制造程序，资源的局限性，无毒性和它们的环境友好性质。μPAD,也被确定为即时医疗设备或医疗保健设备已成功应用于诊断等多个领域，生物，食品安全,环境,电化学和最重要的比色/荧光传感器，由于没有任何外力的分析物的有吸引力的被动运动。近年来,已经报道了大量的比色和荧光探针可以选择性地识别μPAD中的分析物。然而,没有对其结构-活动关系进行有组织的审查。在这次审查中,我们重点总结了μPAD中使用的比色和荧光探针。本文讨论了作为有效μPAD信号单元的各种探针的结构和功能之间的关系。包括纳米材料在内的探针，纳米酶，聚合物和有机分子，还讨论了它们在传感性能方面的结构活性以及检测极限。这篇综述有望帮助读者更好地理解μPAD中使用的各种化学和生物探针的传感机制，以及促进他们在该领域的进步。另一方面,这篇综述还有助于研究人员增强μPAD,并为协同应用现有分子探针作为全球大流行新型冠状病毒COVID-19的有效诊断工具铺平了道路.
