Abstract：
A novel methylotrophic bacterium, strain Zm11T, was isolated from reddish brown snow collected in a moor in Japan. Cells of the isolate were Gram-stain-negative, motile, and rod-shaped (0.6-0.7 × 1.2-2.7 μm). Growth was observed at 5-32 °C with an optimum growth temperature of 25-28 °C. The pH range for growth was 5.4-7.8 with an optimum pH of 6.8. The strain utilized only methanol as carbon and energy sources for aerobic growth. The major cellular fatty acids (> 40% of total) were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16: 0. The predominant quinone was Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The complete genome of strain Zm11T is composed of a circular chromosome (2,800,413 bp), with G + C content of 46.4 mol%. Phylogenetic analyses were conducted based on the 16S rRNA gene sequence and conserved proteins encoded in the genome. The results of analyses indicate that strain Zm11T is a member of the family Methylophilaceae but does not belong to any existing genus. On the basis of its genomic and phenotypic properties, strain Zm11T (= DSM111909T = NBRC114766T) is proposed as the type strain of a new species in a new genus, Methyloradius palustris gen. nov., sp. nov.
摘要：
一种新型的甲基营养细菌，菌株Zm11T，是从日本沼地收集的红棕色雪中分离出来的。分离物的细胞为革兰氏染色阴性，能动,杆状(0.6-0.7×1.2-2.7μm)。在5-32°C观察到生长,最佳生长温度为25-28°C。生长的pH范围为5.4-7.8，最佳pH为6.8。该菌株仅利用甲醇作为有氧生长的碳源和能源。主要细胞脂肪酸(>总量的40%)为特征3(C16:1ω7c和/或C16:1ω6c)和C16:0。主要的醌是Q-8，主要的极性脂质是磷脂酰乙醇胺和磷脂酰甘油。Zm11T菌株的完整基因组由环状染色体(2,800,413bp)组成，G+C含量为46.4mol%。基于16SrRNA基因序列和基因组中编码的保守蛋白质进行系统发育分析。分析结果表明，菌株Zm11T是嗜甲基科的成员，但不属于任何现有属。根据其基因组和表型特性，菌株Zm11T（=DSM111909T=NBRC114766T）被提议作为新属中新物种的类型菌株，太平洋甲流。11月。,sp.11月。
