Abstract：
The presence of anaerobic microflora on fresh beef carcass and rump steaks, which may contribute to meat spoilage, was explored in this study. A total of 120 carcass and 120 rump steak swabs were collected immediately after slaughtering and boning, respectively from five meat plants, anaerobically incubated and enriched at 4°C for 3 weeks. This was followed by DNA extraction and 16S rRNA amplicon sequencing using the Illumina MiSeq, with subsequent bioinformatics analysis. The enriched microbiota of the samples was classified and grouped into 149 operational taxonomic units (OTUs). The microbiota recovered from both sample types consisted mainly of Carnobacterium, with an average relative abundance of 28.4% and 32.8% in beef carcasses and beef rump steaks, respectively. This was followed by Streptococcus, Serratia, Lactococcus, Enterococcus, Escherichia-Shigella, Raoultella and Aeromonas ranging from 1.5 to 20% and 0.1 to 29.8% in enriched carcasses and rump steak swabs, respectively. Trichococcus, Bacteroides, Dysgomonas, Providencia, Paraclostridium and Proteus were also present ranging from 0 to 0.8% on carcass and 0 to 1.8% on rump steak swabs, respectively. Alpha and beta diversity measurements showed limited diversity between the two sample types, but some differences between samples from the beef plants investigated were evident. This study highlights the presence of potential spoilage bacteria, mainly anaerobic genera on and between carcass and rump steaks, as an indication of contamination on and between these samples.
摘要：
新鲜牛肉尸体和臀部牛排上存在厌氧微生物，这可能会导致肉类变质，在这项研究中进行了探索。在屠宰及去骨后立即收集共120具屠体及120份臀部牛排拭子,分别来自五种肉类植物，厌氧孵育并在4°C富集3周。随后使用IlluminaMiSeq进行DNA提取和16SrRNA扩增子测序，随后的生物信息学分析。样品的富集微生物群被分类并分组为149个操作分类单位(OTU)。从两种样品类型中回收的微生物群主要由肉菌组成，牛肉尸体和牛肉臀部牛排的平均相对丰度为28.4%和32.8%，分别。其次是链球菌，Serratia,乳球菌,肠球菌,大肠杆菌志贺氏菌,丰富的尸体和臀部牛排拭子中的Raoultella和Aeromonas范围为1.5至20％和0.1至29.8％，分别。毛球菌,拟杆菌,Dysgomonas,普罗维登西亚,在屠体上也有0-0.8%，在臀部牛排拭子上有0-1.8%。分别。α和β多样性测量显示两种样本类型之间的多样性有限，但是所调查的牛肉植物的样品之间存在一些差异。这项研究强调了潜在腐败细菌的存在，主要是在car体和臀部牛排上和之间的厌氧属，作为这些样品上和样品之间污染的迹象。
