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Abstract：
The characterization of plant compounds with pharmacological activity is a field of great relevance in research and development. As such, identification techniques with the goal of developing new drugs or even validating the bioactive properties of extracts must be explored in order to further expand the knowledge of plant extract composition. Most works in this field employ HPLC, when exploring non-structural and cell wall carbohydrates from Rhynchelytrum repens. Phenolic compounds were studied by classical chromatography techniques and UV-vis spectrophotometry, with C-glycosylated flavonoids being detected but with no further details regarding the chemical structure of these compounds. In this work we employ paper spray ionization mass spectrometry (PS-MS) for the evaluation of the chemical profile of R. repens methanol extract. Positive ionization mode identified 15 compounds, belonging to flavonoids, fatty acids, and other classes of compounds; negative mode ionization was able to identify 20 compounds comprising the classes of quinic acids, stilbenes and flavonoids. PS-MS proved effective for the evaluation of R. repens extracts, making it possible to identify a total of thirty-five compounds. The bioactive properties attributed to R. repens were confirmed by the identification and characterization of compounds identified by PS-MS.
摘要：
具有药理活性的植物化合物的表征是研究和开发中非常重要的领域。因此，为了进一步扩大植物提取物成分的知识，必须探索以开发新药物甚至验证提取物生物活性特性为目标的鉴定技术。该领域的大多数工作都采用HPLC，当探索来自Rhynchelytrum的非结构和细胞壁碳水化合物时。酚类化合物通过经典的色谱技术和紫外-可见分光光度法进行了研究，检测到C-糖基化的类黄酮，但没有关于这些化合物的化学结构的进一步细节。在这项工作中，我们采用纸喷雾电离质谱（PS-MS）来评估R.repens甲醇提取物的化学特征。正离子模式鉴定出15种化合物,属于类黄酮，脂肪酸，和其他类别的化合物；负模式电离能够识别20种包含奎尼酸类别的化合物，二苯乙烯和类黄酮。PS-MS被证明对R.repens提取物的评估有效，使鉴定总共35种化合物成为可能。由PS-MS鉴定的化合物的鉴定和表征证实了归因于R.repens的生物活性。
