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Abstract：
With the rise of long-read sequencers and long-range technologies, delivering high-quality plant genome assemblies is no longer reserved to large consortia. Not only sequencing techniques, but also computer algorithms have reached a point where the reconstruction of assemblies at the chromosome scale is now feasible at the laboratory scale. Current technologies, in particular long-range technologies, are numerous, and selecting the most promising one for the genome of interest is crucial to obtain optimal results. In this study, we resequenced the genome of the yellow sarson, Brassica rapa cv. Z1, using the Oxford Nanopore PromethION sequencer and assembled the sequenced data using current assemblers. To reconstruct complete chromosomes, we used and compared three long-range scaffolding techniques, optical mapping, Omni-C, and Pore-C sequencing libraries, commercialized by Bionano Genomics, Dovetail Genomics, and Oxford Nanopore Technologies, respectively, or a combination of the three, in order to evaluate the capability of each technology.
摘要：
随着长读测序仪和远程技术的兴起，提供高质量的植物基因组组装不再保留给大型财团。不仅仅是测序技术,而且，计算机算法已经达到了在实验室规模上现在可以在染色体规模上重建组件的地步。当前技术,特别是远程技术，很多,和选择最有前途的一个感兴趣的基因组是至关重要的，以获得最佳结果。在这项研究中,我们对黄萨森的基因组进行了重新测序，芸苔属拉帕简历。Z1，使用牛津纳米孔PromethION测序仪，并使用当前的汇编器组装测序数据。为了重建完整的染色体，我们使用并比较了三种远程脚手架技术，光学测绘,Omni-C,和Pore-C测序文库，由Bionano基因组学商业化，燕尾基因组学，和牛津纳米孔技术，分别,或者三者的组合，以评估每种技术的能力。
